Heparin-poloxamer hydrogel-encapsulated rhFGF21 enhances wound healing in diabetic mice.

Abstract

Wound healing, especially for diabetic wounds, is a lengthy and complicated process involving interactions and responses at the protein, cell, and tissue levels. Loading of growth factors into a hydrogel to construct a sustained-release system is considered a promising approach to improve wound healing. The present study investigates the effect of thermosensitive heparin-poloxamer (HP) hydrogel-encapsulated recombinant human fibroblast growth factor 21 (rhFGF21) on wound healing in mice with streptozotocin-induced diabetes mellitus. First, we studied the in vitro release of rhFGF21 from the rhFGF21-HP coacervate. The results showed that HP might control the release of rhFGF21. Next, we examined the effect of rhFGF21-HP on skin wound healing in diabetic mice. Our data showed that rhFGF21-HP significantly improved wound closure; promoted granulation, collagen deposition, and re-epithelialization; and enhanced the expression of CD31. Moreover, rhFGF21-HP had obvious advantages in diabetic wound healing. Therefore, the results suggest that the rhFGF21-HP hydrogel polymer plays an important role in skin wound healing. This work provides a suitable sustained-release delivery system that can continuously release rhFGF21 and presents a promising therapeutic strategy for wound healing in patients with diabetes.-Liu, H., Zhao, Y., Zou, Y., Huang, W., Zhu, L., Liu, F., Wang, D., Guo, K., Hu, J., Chen, J., Ye, L., Li, X., Lin, L. Heparin-poloxamer hydrogel-encapsulated rhFGF21 enhances wound healing in diabetic mice.

• Authors: Huan Liu, Yeli Zhao, Yuchi Zou, Wenting Huang, Liyun Zhu, Fei Liu, Dongxue Wang, Kaiming Guo, Jian Hu, Jun Chen, Lixia Ye, Xiaokun Li, Li Lin
• Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (FASEB J) Vol. 33 Issue 9 Pg. 9858-9870 (09 2019) ISSN: 1530-6860 [Electronic] United States
• PMID: 31166803 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Dosage Forms
• Hydrogels
• Insulin
• Recombinant Proteins
• fibroblast growth factor 21
• Poloxamer
• Fibroblast Growth Factors
• Heparin

Topics

• Animals
• Blood Glucose
• Diabetes Mellitus, Experimental
• Dosage Forms
• Drug Liberation
• Fibroblast Growth Factors (administration & dosage, chemistry, pharmacology)
• Glucose Tolerance Test
• Heparin (chemistry)
• Humans
• Hydrogels (chemistry)
• Insulin
• Male
• Mice
• Mice, Inbred C57BL
• Poloxamer (chemistry)
• Recombinant Proteins
• Wound Healing (drug effects)