Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry.

Abstract

Several Ebola viruses cause outbreaks of lethal haemorrhagic fever in humans, but developing therapies tackle only Zaire Ebola virus. Dendritic cells (DCs) are targets of this infection in vivo. Here, we found that Ebola virus entry into activated DCs requires the sialic acid-binding Ig-like lectin 1 (Siglec-1/CD169), which recognizes sialylated gangliosides anchored to viral membranes. Blockage of the Siglec-1 receptor by anti-Siglec-1 monoclonal antibodies halted Ebola viral uptake and cytoplasmic entry, offering cross-protection against other ganglioside-containing viruses such as human immunodeficiency virus type 1.

• Authors: Daniel Perez-Zsolt, Itziar Erkizia, Maria Pino, Mónica García-Gallo, Maria Teresa Martin, Susana Benet, Jakub Chojnacki, María Teresa Fernández-Figueras, Dolores Guerrero, Victor Urrea, Xabier Muñiz-Trabudua, Leonor Kremer, Javier Martinez-Picado, Nuria Izquierdo-Useros
• Journal: Nature microbiology (Nat Microbiol) Vol. 4 Issue 9 Pg. 1558-1570 (09 2019) ISSN: 2058-5276 [Electronic] England
• PMID: 31160823 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Gangliosides
• Interferon-alpha
• Lipopolysaccharides
• SIGLEC1 protein, human
• Sialic Acid Binding Ig-like Lectin 1

Topics

• Antibodies, Monoclonal (pharmacology)
• Cytoplasm (virology)
• Dendritic Cells (drug effects, metabolism, virology)
• Ebolavirus (physiology)
• Gangliosides (metabolism)
• HIV-1 (physiology)
• Hemorrhagic Fever, Ebola (virology)
• Host-Pathogen Interactions (drug effects)
• Humans
• Interferon-alpha (pharmacology)
• Lipopolysaccharides (pharmacology)
• Sialic Acid Binding Ig-like Lectin 1 (antagonists & inhibitors, immunology, metabolism)
• Virion (metabolism)
• Virus Attachment (drug effects)
• Virus Internalization (drug effects)