Proximity hybridization-induced on particle DNA walker for ultrasensitive protein detection.
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| Abstract |
A simple proximity hybridization-induced on particle DNA walker was designed for ultrasensitive detection of proteins, for example platelet-derived growth factor (PDGF-BB) secreted by cancer cells, in which the DNA walker was activated by specific target binding and powered by an enzymatic cleavage to produce amplified signal. High-density FAM-labeled hairpin oligonucleotides (FAM-DNA1) were functionalized on AuNPs to construct three-dimensional (3D) DNA tracks. The specific binding of PDGF-BB with two aptamer probes (DNA3 and DNA4) led to the proximity hybridization-induced DNA displacement and the free of DNA walker (DNA2) to perform movement on the 3D tracks by an enzymatic cleavage, resulting in the release of massive FAM-DNA1 fragments from the AuNPs and the generation of fluorescent signal. This DNA walker based sensing strategy could detect PDGF-BB in a concentration range of 4 orders of magnitude with a detection limit down to sub-pM level. The practical applicability of the assay was demonstrated by detecting PDGF-BB secreted from MCF-7 cells with satisfactory results. The proposed DNA walker based assay could conveniently detect PDGF-BB with high sensitivity and good accuracy, along with the good extensibility of the assay, showing promise for practical diagnosis.
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| Authors | Haiying Gan, Jie Wu, Huangxian Ju |
| Journal | Analytica chimica acta (Anal Chim Acta) Vol. 1074 Pg. 142-149 (Oct 03 2019) ISSN: 1873-4324 [Electronic] Netherlands |
| PMID | 31159934 (Publication Type: Journal Article) |
| Copyright | Copyright © 2019 Elsevier B.V. All rights reserved. |
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