Transaminase-Catalyzed Continuous Synthesis of Biogenic Aldehydes.

Abstract

The physiological role of biogenic aldehydes, such as 3,4-dihydroxyphenylacetaldehyde (DOPAL), has been associated with cardiovascular and neurodegenerative disorders. The availability of these substrates is limited and robust synthetic methodologies would greatly facilitate further biological studies. Herein, a transaminase-mediated single-step process in continuous mode, which leads to excellent product yields (90-95 %), is reported. Coimmobilization of the pyridoxal phosphate (PLP) cofactor eliminated the need for exogenous addition of this reagent without affecting the longevity of the system, delivering a truly self-sufficient process.

• Authors: Martina L Contente, Francesca Paradisi
• Journal: Chembiochem : a European journal of chemical biology (Chembiochem) Vol. 20 Issue 22 Pg. 2830-2833 (11 18 2019) ISSN: 1439-7633 [Electronic] Germany
• PMID: 31158309 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Aldehydes
• Amines
• Bacterial Proteins
• Pyridoxal Phosphate
• Transaminases

Topics

• Aldehydes (chemical synthesis)
• Amines (chemistry)
• Bacterial Proteins (chemistry)
• Biocatalysis
• Halomonas (enzymology)
• Pyridoxal Phosphate (chemistry)
• Transaminases (chemistry)