Physiological Effects and Organ Distribution of Bacillus amyloliquefaciens AS385 Culture Broth Powder Containing 1-Deoxynojirimycin in C57BL/6J Mice.

Abstract

1-Deoxynojirimycin (DNJ) has been known as a potent α-glucosidase inhibitor from mulberry leaves and considered beneficial in prevention of type 2 diabetes. Due to limited amount of DNJ in mulberry leaves, recent studies have focused in finding alternative source that can produce higher amount of DNJ. Previously, we produced a high DNJ-containing culture medium from Bacillus amyloliquefaciens AS385 and constructed a concentration method of bacterial culture medium using cation exchange column. However, less complicated concentration procedure is necessary to save time and cost during the large-scale production. Therefore, we developed a simpler concentration method using anion exchange resin to yield B. amyloliquefaciens AS385 culture broth powder (CBP; 1% DNJ) and evaluated the physiological effects of 5-wk dietary CBP intake in C57BL/6J mice. CBP intake tended to suppress the elevation of blood glucose level during oral glucose tolerance test. Moreover, CBP intake significantly lowered the fasting plasma glucose level and white adipose tissue mass. Next, we evaluated the absorption and distribution of DNJ in mice organs after daily CBP intake. We found detectable amount of DNJ in organs with intestine and kidney as the major targeted organs. We concluded that the DNJ content in CBP is absorbed from digestive tract, distributed and accumulated in organs, which most likely to contribute to the alteration of blood glucose regulation and adiposity in C57BL/6J mice. Our study was the first to report the physiological effects of CBP produced from B. amyloliquefaciens AS385 and the organ distribution of DNJ from CBP.

• Authors: Isabella Supardi Parida, Soo Takasu, Junya Ito, Ryoichi Ikeda, Kenji Yamagishi, Toshiyuki Kimura, Teruo Miyazawa, Takahiro Eitsuka, Kiyotaka Nakagawa
• Journal: Journal of nutritional science and vitaminology (J Nutr Sci Vitaminol (Tokyo)) Vol. 65 Issue 2 Pg. 157-163 ( 2019) ISSN: 1881-7742 [Electronic] Japan
• PMID: 31061284 (Publication Type: Journal Article)

Chemical References

• Blood Glucose
• Culture Media
• Insulin
• Powders
• 1-Deoxynojirimycin

Topics

• 1-Deoxynojirimycin (metabolism, pharmacokinetics, pharmacology)
• Animals
• Bacillus amyloliquefaciens (metabolism)
• Blood Glucose (analysis, drug effects)
• Body Weight (drug effects)
• Culture Media
• Insulin (blood)
• Male
• Mice
• Mice, Inbred C57BL
• Powders
• Tissue Distribution