Abstract |
Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-κB using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-κB. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-κB (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application.
|
Authors | Adriano Costa de Camargo, Aline Camarão Telles Biasoto, Andrés R Schwember, Daniel Granato, Gabriela Boscariol Rasera, Marcelo Franchin, Pedro L Rosalen, Severino Matias Alencar, Fereidoon Shahidi |
Journal | Food chemistry
(Food Chem)
Vol. 290
Pg. 229-238
(Aug 30 2019)
ISSN: 1873-7072 [Electronic] England |
PMID | 31000041
(Publication Type: Journal Article)
|
Copyright | Copyright © 2019 Elsevier Ltd. All rights reserved. |
Chemical References |
- Antioxidants
- Lipopolysaccharides
- NF-kappa B
- Phenols
- Plant Extracts
|
Topics |
- Animals
- Antioxidants
(chemistry, pharmacology)
- Cell Survival
(drug effects)
- Chromatography, High Pressure Liquid
- Colorimetry
- Lipopolysaccharides
(toxicity)
- Macrophages
(cytology, drug effects, metabolism)
- Mice
- NF-kappa B
(metabolism)
- Phenols
(chemistry, pharmacology)
- Plant Extracts
(chemistry)
- Principal Component Analysis
- RAW 264.7 Cells
- Vitis
(chemistry, metabolism)
|