Nitidine Chloride inhibits cell proliferation and invasion via downregulation of YAP expression in prostate cancer cells.

Abstract

Nitidine chloride (NC) exhibits tumor suppressive function in a variety of human cancers. However, the molecular mechanism of NC-triggered anti-cancer activity has not been fully elucidated. In the present study, we aim to investigate the anti-tumor molecular basis of NC in prostate cancer cells. Multiple approaches including MTT, FACS, wound healing assay, Transwell invasion assay, Transfection, and Western blotting were performed. We found that NC inhibited cell growth and induced apoptosis in prostate cancer cells. Moreover, NC suppressed cell migration and invasion in prostate cancer cells. Notably, we found that NC decreased the expression of YAP oncoprotein in prostate cancer cells. Downregulation of YAP enhanced the anti-tumor function mediated by NC in prostate cancer cells. On the contrary, upregulation of YAP abrogated the anti-cancer activity of NC treatment in prostate cancer cells. Our findings indicate that NC could be useful as a YAP inhibitor for the treatment of prostate cancer cells.

• Authors: Ying Shi, Tong Cao, Yu Sun, Jun Xia, Peter Wang, Jia Ma
• Journal: American journal of translational research (Am J Transl Res) Vol. 11 Issue 2 Pg. 709-720 ( 2019) ISSN: 1943-8141 [Print] United States
• PMID: 30899373 (Publication Type: Journal Article)