Ameliorative effect of certolizumab on experimentally induced acute necrotic pancreatitis in rats.

Abstract

OBJECTIVE:

The effects of Certolizumab, a pegylated monoclonal antibody to tumor necrosis factor α, on experimentally induced acute pancreatitis (AP) were examined.

METHODS:

Thirty-six Wistar Albino male rats were randomly divided into four groups. Group I was the control group and no medication administered to this group. Group II was the Certolizumab group, and 100 ml/kg serum physiologic administered into the biliopancreatic duct and a single dose of 10 μg Certolizumab was simultaneously administered intraperitoneally. Acute pancreatitis was induced with a retrograde injection of 3% Na taurocholate into the common biliopancreatic duct in the study (Group III) and treatment (Groups IV) groups. Rats were sacrificed 72 hours later. Serum amylase, lipase, lactate dehydrogenase activities, along with pancreatic histopathology, were examined.

RESULTS:

Certolizumab treatment significantly decreased serum amylase, lipase, and LDH levels; histopathologically edema, hemorrhage, parenchymal necrosis, fat necrosis, and infiltration scores; immunohistochemically MDA, MPO, TNF-α and Caspase-3 activity.

CONCLUSION:

The results support the idea that certolizumab might be beneficial for the severity of AP.

• Authors: Mehmet Ali Kosekli, Özkan Herek, Özlem Ozmen, Sima Sahinduran
• Journal: Revista da Associacao Medica Brasileira (1992) (Rev Assoc Med Bras (1992)) Vol. 65 Issue 2 Pg. 204-210 (Feb 2019) ISSN: 1806-9282 [Electronic] Brazil
• PMID: 30892445 (Publication Type: Journal Article)

Chemical References

• Immunosuppressive Agents
• Taurocholic Acid
• Certolizumab Pegol

Topics

• Animals
• Certolizumab Pegol (therapeutic use)
• Disease Models, Animal
• Immunosuppressive Agents (therapeutic use)
• Male
• Pancreatitis, Acute Necrotizing (chemically induced, drug therapy, pathology)
• Rats
• Rats, Wistar
• Taurocholic Acid