Abstract |
Systems biology is increasingly being applied in nanosafety research for observing and predicting the biological perturbations inflicted by exposure to nanoparticles (NPs). In the present study, we used a combined transcriptomics and proteomics approach to assess the responses of human monocytic cells to Au-NPs of two different sizes with three different surface functional groups, i.e., alkyl ammonium bromide, alkyl sodium carboxylate, or poly( ethylene glycol) (PEG)-terminated Au-NPs. Cytotoxicity screening using THP-1 cells revealed a pronounced cytotoxicity for the ammonium-terminated Au-NPs, while no cell death was seen after exposure to the carboxylated or PEG-modified Au-NPs. Moreover, Au-NR3+ NPs, but not the Au-COOH NPs, were found to trigger dose-dependent lethality in vivo in the model organism, Caenorhabditis elegans. RNA sequencing combined with mass spectrometry-based proteomics predicted that the ammonium-modified Au-NPs elicited mitochondrial dysfunction. The latter results were validated by using an array of assays to monitor mitochondrial function. Au-NR3+ NPs were localized in mitochondria of THP-1 cells. Moreover, the cationic Au-NPs triggered autophagy in macrophage-like RFP-GFP-LC3 reporter cells, and cell death was aggravated upon inhibition of autophagy. Taken together, these studies have disclosed mitochondria-dependent effects of cationic Au-NPs resulting in the rapid demise of the cells.
|
Authors | Audrey Gallud, Katharina Klöditz, Jimmy Ytterberg, Nataliya Östberg, Shintaro Katayama, Tiina Skoog, Vladimir Gogvadze, Yu-Zen Chen, Ding Xue, Sergio Moya, Jaime Ruiz, Didier Astruc, Roman Zubarev, Juha Kere, Bengt Fadeel |
Journal | Scientific reports
(Sci Rep)
Vol. 9
Issue 1
Pg. 4366
(03 13 2019)
ISSN: 2045-2322 [Electronic] England |
PMID | 30867451
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Ammonium Compounds
- Cations
- Proteome
- Gold
|
Topics |
- Ammonium Compounds
(chemistry)
- Autophagy
(drug effects)
- Cations
(chemistry, pharmacology)
- Cell Death
(drug effects)
- Cell Line, Tumor
- Chemical Phenomena
- Dose-Response Relationship, Drug
- Gene Expression Profiling
- Gold
(chemistry, pharmacology)
- Humans
- Metabolic Networks and Pathways
- Metal Nanoparticles
(chemistry, ultrastructure)
- Mitochondria
(drug effects, genetics, metabolism, ultrastructure)
- Oxidative Phosphorylation
- Proteome
- Proteomics
(methods)
- Transcriptome
|