XB130 is an adaptor
protein that functions as a mediator of multiple
tyrosine kinases important for regulating cell proliferation, survival, migration and invasion. Formerly predicted as an oncogene, alterations of its expression are documented in various human
cancers. However, the exact role of XB130 in
tumorigenesis is unknown. To address its function in skin
tumorigenesis, a two-stage dimethylbenzanthracene (DMBA)/12-O-tetradecanoylphorbol 13-acetate (TPA) study was performed on XB130 knockout (KO), heterozygous (HZ) and wild-type (WT) littermate mice. DMBA/TPA-treated XB130 KO and HZ males developed a significantly higher number of epidermal
tumors that were notably larger in size than did WT mice. Interestingly, DMBA/TPA-treated female mice did not show any difference in
tumor multiplicity regardless of the genotypes. The skin
tumor lesions of XB130 KO males were more progressed with an increased frequency of
keratoacanthoma. Deficiency of XB130 dramatically increased epidermal
tumor cell proliferation. The responses to DMBA and TPA stimuli were also individually investigated to elucidate the mechanistic role of XB130 at different stages of
tumorigenesis. DMBA-treated male XB130 KO mice showed compensatory p53-mediated stress response. TPA-treated XB130 KO males demonstrated more skin ulceration with more severe
edema, enhanced cell proliferation, accumulation of infiltrating neutrophils and increased production of pro-inflammatory
cytokine genes compared with WT mice. Enhanced activities of
nuclear factor-kappa B pathway, increased
protein expression of metalloproteinase-9 and ERK1/2 phosphorylation were found in these KO mice. These findings demonstrate that XB130 acts as a
tumor suppressor in
carcinogen-induced skin
tumorigenesis that may be mediated through inhibiting
inflammation.