Abstract | BACKGROUND & AIMS:
Food additives, such as emulsifiers, stabilizers, or bulking agents, are present in the Western diet and their consumption is increasing. However, little is known about their potential effects on intestinal homeostasis. In this study we examined the effect of some of these food additives on gut inflammation. METHODS: RESULTS: Diets enriched in MDX, but not propylene glycol or animal gelatin, exacerbated intestinal inflammation in both models. Analysis of the mechanisms underlying the detrimental effect of MDX showed up-regulation of inositol requiring protein 1β, a sensor of ER stress, in goblet cells, and a reduction of mucin-2 expression with no significant change in mucosa-associated microbiota. Stimulation of murine intestinal crypts and HT29-methotrexate treated cell line cells with MDX induced inositol requiring protein 1β via a p38 MAP kinase-dependent mechanism. Treatment of mice with TUDCA prevented mucin-2 depletion and attenuated colitis in MDX-fed mice. CONCLUSIONS: MDX increases ER stress in gut epithelial cells with the downstream effect of reducing mucus production and enhancing colitis susceptibility.
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Authors | Federica Laudisi, Davide Di Fusco, Vincenzo Dinallo, Carmine Stolfi, Antonio Di Grazia, Irene Marafini, Alfredo Colantoni, Angela Ortenzi, Claudia Alteri, Francesca Guerrieri, Maria Mavilio, Francesca Ceccherini-Silberstein, Massimo Federici, Thomas Thornton MacDonald, Ivan Monteleone, Giovanni Monteleone |
Journal | Cellular and molecular gastroenterology and hepatology
(Cell Mol Gastroenterol Hepatol)
Vol. 7
Issue 2
Pg. 457-473
( 2019)
ISSN: 2352-345X [Electronic] United States |
PMID | 30765332
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Food Additives
- Membrane Proteins
- Polysaccharides
- maltodextrin
- Ern2 protein, mouse
- Protein Serine-Threonine Kinases
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Cattle
- Colitis
(microbiology, pathology)
- Diet
- Disease Progression
- Endoplasmic Reticulum Stress
(drug effects)
- Epithelial Cells
(drug effects, pathology)
- Food Additives
(adverse effects)
- Gastrointestinal Microbiome
- Inflammation
(microbiology, pathology)
- Intestines
(microbiology, pathology)
- Membrane Proteins
(metabolism)
- Mice, Inbred BALB C
- Mucus
(metabolism)
- Polysaccharides
(adverse effects)
- Protein Serine-Threonine Kinases
(metabolism)
- Swine
- Unfolded Protein Response
(drug effects)
- Up-Regulation
(drug effects)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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