Unconventional
prefoldin RPB5 interactor (URI, or RMP, a member of the
prefoldin family of
molecular chaperones) exhibits oncogenic activity in several types of
cancer, including
ovarian cancer. However, the underlying regulatory mechanism in
ovarian cancer remains unclear.
MicroRNAs (
miRNAs) negatively regulate gene expression, and their dysregulation has been implicated in
tumorigenesis. To elucidate the role of
miRNAs in URI-induced
ovarian cancer, miR-598 and URI were overexpressed in the SKOV3
ovarian cancer cell line. The CCK8 kit was used to determine cell proliferation, and the Transwell assay was used to measure cell invasion and migration. RT-PCR and western blotting were used to analyze the expression of miR-598 and URI, and the
luciferase reporter assay was used to examine the interaction between miR-598 and URI. Nude mice were used to characterize the regulation of
tumor growth in vivo. The results showed that the expression of miR-598 inhibited the proliferation, invasion, and migration of
ovarian cancer cells by targeting URI. The inhibitory effect of miR-598 was reversed by overexpression of URI. The
luciferase reporter assay showed that miR-598 downregulated URI by directly targeting the
3' UTR of URI. In vivo studies showed that the expression of miR-598 significantly inhibited the growth of
tumors. Taken together, the results suggested that miR-598 inhibited
tumor growth and
metastasis by targeting URI.