miR-98 acts as an inhibitor in chronic constriction injury-induced neuropathic pain via downregulation of high-mobility group AT-hook 2.

Abstract

Neuropathic pain, resulting from somatosensory nervous system dysfunction, remains a serious public health problem worldwide. microRNAs are involved in the physiological processes of neuropathic pain. However, the biological roles of miR-98 in neuropathic pain development have not been investigated. Therefore, in our current study, we focused on the effects of miR-98 in neuropathic pain. It was shown that miR-98 was significantly downregulated in chronic sciatic nerve injury (CCI) rat models. In addition, high mobility group A2 (HMGA2) was obviously upregulated in CCI rats. Overexpression of miR-98 inhibited neuropathic pain progression, including mechanical and thermal hyperalgesia. By a bioinformatics analysis, HMGA2 was predicted as a direct target of miR-98. The negative correlation between miR-98 and HMGA2 was validated in our present study. Furthermore, overexpression of miR-98 dramatically repressed HMGA2 protein and messenger RNA (mRNA) expression. Neuroinflammation participates in neural-immune interactions, which can contribute to the neuropathic pain development. Meanwhile, we found that inflammatory cytokine (interleukin [IL]-6, IL-1β, and COX-2) protein expression in rats infected with LV-miR-98 was greatly suppressed. Taking these results together, we concluded that miR-98 might depress neuropathic pain development through modulating HMGA2.

• Authors: Yang Zhang, Zhen Su, Li-Jun An, Lin Li, Meng Wei, Dong-Jian Ge, Hai-Lin Liu
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 120 Issue 6 Pg. 10363-10369 (06 2019) ISSN: 1097-4644 [Electronic] United States
• PMID: 30659647 (Publication Type: Journal Article)
• Copyright: © 2019 Wiley Periodicals, Inc.

Chemical References

• Cytokines
• HMGA2 Protein
• HMGA2 protein, rat
• MIRN98 microRNA-98, rat
• MicroRNAs

Topics

• Animals
• Constriction, Pathologic (complications)
• Cytokines (metabolism)
• Gene Expression Regulation
• HMGA2 Protein (antagonists & inhibitors, genetics, metabolism)
• Hyperalgesia (complications)
• Inflammation (etiology, pathology, prevention & control)
• Male
• MicroRNAs (genetics)
• Neuralgia (etiology, pathology, prevention & control)
• Peripheral Nerve Injuries (complications)
• Rats
• Rats, Sprague-Dawley
• Sciatic Nerve (injuries)