In vivo biocompatibility of an interim denture resilient liner containing antifungal drugs.

Abstract

STATEMENT OF PROBLEM:

Antifungal agents incorporated into interim denture resilient liners have been suggested as an adjunct treatment for denture stomatitis (DS). However, before applying this protocol to humans, biocompatibility analysis of such drugs in animal models is required.

PURPOSE:

The purpose of this animal study was to evaluate the in vivo biocompatibility of an interim resilient liner modified with minimum inhibitory concentrations (MICs) of antifungal drugs for Candida albicans biofilm.

MATERIAL AND METHODS:

Sixty Wistar rats were divided into 6 groups (n=5): PC=positive control/no protocol; IOD (intraoral device)=rats using an acrylic resin palatal device (PD); Tru=rats using a PD relined with Trusoft; and Ny (nystatin), Chx (chlorhexidine diacetate), and Ke (ketoconazole) groups=rats using a PD relined with Trusoft + drug MICs. The rats were sacrificed at 7 or 14 days of trial. Histopathological qualitative analysis was performed by comparing photomicrographs of histological sections of the intermolar region. Morphological changes in the epithelium and keratin were quantitatively analyzed by computerized planimetry, and data were analyzed by using 2-way ANOVA and the Tukey HSD test (α=.05).

RESULTS:

Quantitative analysis showed that only PD containing Ke significantly decreased the thickness and area of the keratin compared with the other groups (P<.001), which showed no differences between each other (P>.05). These results agreed with those of qualitative analysis.

CONCLUSIONS:

Incorporation of MICs of Ny and Chx in Trusoft did not induce histopathological changes in the rat palatal mucosa, suggesting the in vivo biocompatibility of this DS treatment.

• Authors: Juliana Hotta, Gustavo Pompermeier Garlet, Tania Mary Cestari, Jozely Francisca Mello Lima, Vinícius Carvalho Porto, Vanessa Migliorini Urban, Karin Hermana Neppelenbroek
• Journal: The Journal of prosthetic dentistry (J Prosthet Dent) Vol. 121 Issue 1 Pg. 135-142 (Jan 2019) ISSN: 1097-6841 [Electronic] United States
• PMID: 30646999 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.

Chemical References

• Acrylic Resins
• Antifungal Agents
• Nystatin
• Keratins
• Chlorhexidine
• Ketoconazole

Topics

• Acrylic Resins
• Analysis of Variance
• Animals
• Antifungal Agents (administration & dosage)
• Biofilms (drug effects)
• Candida albicans (drug effects)
• Chlorhexidine (administration & dosage)
• Denture Liners
• Keratins (drug effects)
• Ketoconazole (administration & dosage)
• Materials Testing
• Microbial Sensitivity Tests
• Mouth Mucosa (cytology, drug effects)
• Nystatin (administration & dosage)
• Rats
• Rats, Wistar