Effects of Cold Atmospheric Plasma on the Expression of Chemokines, Growth Factors, TNF Superfamily Members, Interleukins, and Cytokines in Human Osteosarcoma Cells.

Abstract

BACKGROUND/AIM:

Therapeutic options for osteosarcoma (OS) are still limited. Cold atmospheric plasma (CAP) leads to inhibition of tumor growth and metastasis, but underlying mechanisms are not fully understood. The aim of this study was to investigate CAP-induced changes in cytokine expression in OS cells.

MATERIALS AND METHODS:

OS cell lines (U2-OS, MNNG/HOS) were treated with CAP and administered to an RT2 Profiler PCR Array (Qiagen, Hilden, Germany) detecting 84 chemokines, growth factors, TNF superfamily members, interleukins, and cytokines.

RESULTS:

The analyses showed that 15 factors (C5, CCL5, CNTF, CSF1, CSF3, CXCL1, IL-1A, IL-1B, IL-18, IL-22, IL23A, MSTN, NODAL, TGFβ2, THPO) were induced, but only one factor (VEGFA) was suppressed after CAP treatment.

CONCLUSION:

No extensive systemic cell response with presumably far-reaching consequences for neighboring cells was detectable after CAP treatment. Since the antitumoral effect of CAP on OS cells has already been demonstrated, intraoperative treatment with CAP represents a promising and systemic safe option for the therapy of OS.

• Authors: Lyubomir Haralambiev, Lasse Wien, Nadine Gelbrich, Axel Kramer, Alexander Mustea, Martin Burchardt, Axel Ekkernkamp, Matthias B Stope, Denis Gümbel
• Journal: Anticancer research (Anticancer Res) Vol. 39 Issue 1 Pg. 151-157 (Jan 2019) ISSN: 1791-7530 [Electronic] Greece
• PMID: 30591452 (Publication Type: Journal Article)
• Copyright: Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Cytokines
• Intercellular Signaling Peptides and Proteins
• Interleukins
• Plasma Gases

Topics

• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cytokines (genetics)
• Gene Expression Regulation, Neoplastic (radiation effects)
• Humans
• Intercellular Signaling Peptides and Proteins (genetics)
• Interleukins (genetics)
• Osteosarcoma (genetics, pathology, therapy)
• Plasma Gases (therapeutic use)