[Huotan Jiedu Tongluo decoction alleviates early atherosclerosis of rabbits by inhibiting eNOS uncoupling pathway].

Abstract

The aim of this paper was to study the curative effect of Huotan Jiedu Tongluo (HTJDTL) decoction on a rabbit model with early atherosclerosis (AS),and furtherly to explore whether it could inhibit the BH4/eNOS uncoupling ROS or not. Twenty-four Japanese white rabbits were randomly divided into sham operation group, model group, HTJDTL decoction group and atorvastatin group. Rabbit models with early atherosclerosis were established by high fat diet, nitrogen drying and carotid artery balloon injury. The rabbits were sacrificed at 7th days after balloon injury and several parameters were measured. The pathological morphology of the common carotid artery was observed by HE staining. The blood lipids were detected by peroxidase method. The ratio of vascular eNOS dimer and monomer was measured by Western blot. The ELISA and biochemical technology were respectively used for testing BH4 and ROS levels in serum. The results showed that compared with the sham operation group, the model group had mild stenosis of the common carotid artery lumen, uneven intimal hyperplasia, lipid deposition in the intima and media, and obvious hyperplasia of the adventitia with inflammatory cell infiltration. The HTJDTL decoction could significantly inhibit the intimal hyperplasia compared with the model group, meanwhile, reduce the lipid deposition of the media and the infiltration of the adventitial cells. Compared with the sham operation group, the blood lipids and ROS of the model animals significantly increased, but BH4 and the ratio of eNOS dimer/monomer decreased. Compared with the model group, HTJDTL decoction significantly reduced the TC, ox-LDL and ROS levels, and also up-regulated eNOS dimer/monomer ratio, but it increased BH4 trend without statistical difference. According to the results, it was found that HTJDTL decoction couldsignificantly prevent and improve the vascular remodeling of rabbits model with early atherosclerosis. The mechanism of decoction may largely be related to the inhibition of BH4/eNOS uncoupling and the reduction of oxidative stress.

• Authors: Tong Li, Ming-Jing Zhao, Xiao-Wan Han, Yue Deng, Yi-Zhou Zhao, Tao Yang, Lei Wang, Bao-Fu Wang, Zi-Wen Lu, Wen-Kun Cheng, Ai-Ming Wu, Li-Xia Lou, Jiu-Li Zhao
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 43 Issue 21 Pg. 4305-4310 (Nov 2018) ISSN: 1001-5302 [Print] China
• PMID: 30583633 (Publication Type: Journal Article)
• Copyright: Copyright© by the Chinese Pharmaceutical Association.

Chemical References

• Drugs, Chinese Herbal
• Nitric Oxide Synthase Type III

Topics

• Animals
• Atherosclerosis (drug therapy)
• Carotid Arteries (pathology)
• Drugs, Chinese Herbal (pharmacology)
• Nitric Oxide Synthase Type III (metabolism)
• Oxidative Stress
• Rabbits
• Random Allocation
• Signal Transduction (drug effects)