Urinary Cell-Free DNA: Potential and Applications.

Abstract

Urine could be a convenient source of biomarkers for different diseases and clinical applications, mostly for cancer diagnosis, prognosis, treatment monitoring, and prenatal diagnosis. The ultra-noninvasive sampling and the possibility to analyze large volume are the main undisputed advantages of urine-based protocols. Recent and comprehensive studies showed that urinary cell-free DNA (ucfDNA) is informative to identify the genomic signature of patients, resulting in a huge tool to track the tumor evolution and for personalized medicine in urological and non-urological cancer.In this chapter, we reported the main published evidences on ucfDNA, with the aim at discussing its promising and translatable role in clinical practices.

• Authors: Samanta Salvi, Valentina Casadio
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1909 Pg. 201-209 ( 2019) ISSN: 1940-6029 [Electronic] United States
• PMID: 30580433 (Publication Type: Journal Article)

Chemical References

• Cell-Free Nucleic Acids

Topics

• Animals
• Cell-Free Nucleic Acids (genetics, isolation & purification, urine)
• DNA Copy Number Variations
• Female
• Humans
• Mutation
• Neoplasms (diagnosis, genetics, urine)
• Polymerase Chain Reaction (methods)
• Pregnancy
• Prenatal Diagnosis (methods)
• Prognosis
• Urine Specimen Collection (methods)