Diallyl disulfide potentiates anti-obesity effect of green tea in high-fat/high-sucrose diet-induced obesity.

Abstract

Obesity is a major problem in developed countries and a burden on social health care systems. Several epidemiological studies showed the protective effects of green tea against obesity-related diseases. Cyclic guanosine monophosphate (cGMP) acts as a mediator for the physiological effects of (-)-epigallocatechin-3-O-gallate, the major constituent of green tea. Here, we showed that the level of phosphodiesterase 5, a negative regulator of cGMP, was up-regulated in adipose tissues of high-fat/high-sucrose (HF/HS) diet-fed mice and that this up-regulation was ameliorated by diallyl disulfide (DADS), the major organosulfur in garlic. A green tea extract (GT) and DADS in combination attenuated HF/HS diet-induced adipose increase and triglyceride accumulation in the liver. In these mechanisms, the combination regimen suppressed the HF/HS diet-induced up-regulation of fatty acid synthesis-related enzymes including sterol regulatory element-binding protein-1 (SREBP-1), fatty acid synthase, and stearoyl-CoA desaturase-1. Moreover, this combination diet up-regulated thermogenesis-related genes including peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1 alpha and uncoupling proteins in both white and brown adipose tissues. In conclusion, we identified DADS as an enhancer of the anti-obesity effect of GT accompanied by the suppression of SREBP-1 and activation of PPAR axis. The combination diet is a novel and easily applicable approach against obesity-related diseases.

• Authors: Jaehoon Bae, Motofumi Kumazoe, Yoshinori Fujimura, Hirofumi Tachibana
• Journal: The Journal of nutritional biochemistry (J Nutr Biochem) Vol. 64 Pg. 152-161 (02 2019) ISSN: 1873-4847 [Electronic] United States
• PMID: 30504007 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Chemical References

• Allyl Compounds
• Anti-Obesity Agents
• Disulfides
• Peroxisome Proliferator-Activated Receptors
• Tea
• Sucrose
• diallyl disulfide
• Catechin
• epigallocatechin gallate
• Cyclic Nucleotide Phosphodiesterases, Type 5
• Pde5a protein, mouse

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism)
• Adipose Tissue (drug effects, metabolism)
• Allyl Compounds (pharmacology)
• Animals
• Anti-Obesity Agents (pharmacology)
• Body Weight (drug effects)
• Catechin (analogs & derivatives, pharmacology)
• Cyclic Nucleotide Phosphodiesterases, Type 5 (metabolism)
• Diet, High-Fat (adverse effects)
• Disulfides (pharmacology)
• Gene Expression Regulation (drug effects)
• Lipid Metabolism (drug effects, genetics)
• Male
• Mice
• Mice, Inbred C57BL
• Peroxisome Proliferator-Activated Receptors (metabolism)
• Sucrose (adverse effects)
• Tea