Ivalin Inhibits Proliferation, Migration and Invasion by Suppressing Epithelial Mesenchymal Transition in Breast Cancer Cells.
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| Abstract |
Ivalin, an eudesmane-type sesquiterpene compound, was isolated from the Chinese herb Carpesium divaricatum in our chemistry group. In this study, we investigated the anti-migration and anti-invasion activities and underlying mechanisms of Ivalin in breast cancer cells in vitro. Cell viability was evaluated using the MTT assay, Western blotting was used to determine the expression of E-cadherin, N-cadherin, vimentin and ZEB1, and mRNA levels were analyzed by qPCR. The anti-migration and anti-invasion effects of Ivalin were measured by wound-healing and Transwell assays. In this connection, Ivalin treatment reduced the mRNA and protein expressions of ZEB1 as well as N-cadherin and vimentin expression in various breast cancer cells. E-cadherin expression was enhanced by Ivalin in the same cells, which implied that Ivalin depressed the process of epithelial-to-mesenchymal transition (EMT). Our results revealed that Ivalin significantly inhibited cell proliferation, migration and invasion in breast cancer cells in a dose-dependent manner in vitro. This study suggests that Ivalin may merit further investigation as a potential therapeutic leading compound for the treatment of breast cancer migration and invasion.
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| Authors | Jia-Hui Ma, Jie Qi, Fang-Yuan Liu, Shi-Qi Lin, Cai-Yun Zhang, Wei-Dong Xie, Hang-Yu Zhang, Xia Li |
| Journal | Nutrition and cancer (Nutr Cancer) 2018 Nov-Dec Vol. 70 Issue 8 Pg. 1330-1338 ISSN: 1532-7914 [Electronic] United States |
| PMID | 30463445 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Video-Audio Media) |
| Chemical References |
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