Abstract | BACKGROUND: METHODS: We collected lung tissue at the time of lung transplantation at UCLA from SSc-ILD patients (n = 12) and healthy donors (n = 12); and serum samples from the prospective Oslo University Hospital SSc cohort (n = 292) and healthy donors (n = 100). CX3CL1 was measured by ELISA. Cellular sources of CX3CL1/CX3CR1 in lung tissues were determined by immunohistochemistry and immunofluorescence. ILD progression and new onset PH endpoints were analysed. RESULTS: CX3CL1 concentrations were increased in SSc in lung tissue as well as in sera. In the UCLA cohort, CX3CL1 was highly correlated with DLCO. In the SSc-ILD lungs, CX3CL1 was identified in reactive type II pneumocytes and airway epithelial cells. CX3CR1 stained infiltrating interstitial mononuclear cells, especially plasma cells. In the Oslo cohort, CX3CL1 correlated with anti-Topoisomerase-I-antibody and lung fibrosis. CX3CL1 was associated with ILD progression in multivariable regression analysis but not PH. CONCLUSION: CX3CL1 is associated with progressive SSc-ILD but not SSc-PH. The CX3CR1/CX3CL1- biological axis may be involved in recruiting antibody secreting plasma cells to SSc lungs, thereby contributing to the immune-mediated pathobiology of SSc-ILD.
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Authors | Anna-Maria Hoffmann-Vold, Stephen Samuel Weigt, Vyacheslav Palchevskiy, Elizabeth Volkmann, Rajan Saggar, Ning Li, Øyvind Midtvedt, May Brit Lund, Torhild Garen, Michael C Fishbein, Abbas Ardehali, David J Ross, Thor Ueland, Pål Aukrust, Joseph P Lynch 3rd, Robert M Elashoff, Øyvind Molberg, John A Belperio |
Journal | PloS one
(PLoS One)
Vol. 13
Issue 11
Pg. e0206545
( 2018)
ISSN: 1932-6203 [Electronic] United States |
PMID | 30457999
(Publication Type: Journal Article, Multicenter Study, Observational Study, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- CX3C Chemokine Receptor 1
- CX3CL1 protein, human
- CX3CR1 protein, human
- Chemokine CX3CL1
- SDC1 protein, human
- Syndecan-1
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Topics |
- Adult
- Biomarkers
(metabolism)
- CX3C Chemokine Receptor 1
(metabolism)
- Chemokine CX3CL1
(metabolism)
- Disease Progression
- Female
- Humans
- Hypertension, Pulmonary
(metabolism, pathology, surgery)
- Lung
(metabolism, pathology, surgery)
- Lung Diseases, Interstitial
(metabolism, pathology, surgery)
- Lung Transplantation
- Male
- Middle Aged
- Prospective Studies
- Retrospective Studies
- Scleroderma, Systemic
(metabolism, pathology, surgery)
- Syndecan-1
(metabolism)
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