Cytoprotective Effects of Mesenchymal Stem Cells During Liver Transplantation from Donors After Cardiac Death in Rats.

Abstract

BACKGROUND:

Liver transplantation from donors after cardiac death (DCD) might increase the pool of available organs. Recently, some investigators reported the potential use of mesenchymal stem cells (MSCs) to improve the outcome of liver transplantation from DCD. The aim of this study was to evaluate the cytoprotective effects and safety of MSC transplantation on liver grafts from DCD.

METHODS:

Rats were divided into 4 groups (n = 5) as follows: 1. the heart-beating group, in which liver grafts were retrieved from heart-beating donors; 2. the DCD group, in which liver grafts were retrieved from DCD that had experienced apnea-induced agonal conditions; 3. the MSC-1 group, and 4. the MSC-2 group, in which liver grafts were retrieved as with the DCD group, but were infused MSCs (2.0 × 105 or 1.0 × 106, respectively). The retrieved livers were perfused with oxygenated Krebs-Henseleit bicarbonate buffer (37°C) through the portal vein for 2 hours after 6 hours of cold preservation. Perfusate, bile, and liver tissues were then investigated.

RESULTS:

Bile production in the MSC-2 group was significantly improved compared with that in the DCD group. Based on histologic findings, narrowing of the sinusoidal space in the both MSC groups was improved compared with that in the DCD group.

CONCLUSIONS:

MSCs could protect the function of liver grafts from warm ischemia-reperfusion injury and improve the viability of DCD liver grafts. In addition, we found that the infusion of 1.0 × 106 MSCs does not obstruct the hepatic sinusoids of grafts from DCD.

• Authors: H Sasajima, S Miyagi, Y Kakizaki, T Kamei, M Unno, S Satomi, M Goto
• Journal: Transplantation proceedings (Transplant Proc) Vol. 50 Issue 9 Pg. 2815-2820 (Nov 2018) ISSN: 1873-2623 [Electronic] United States
• PMID: 30401403 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Topics

• Animals
• Death
• Liver (pathology)
• Liver Transplantation (methods)
• Male
• Mesenchymal Stem Cell Transplantation (methods)
• Organ Preservation (methods)
• Rats
• Rats, Wistar
• Reperfusion Injury (pathology, prevention & control)
• Tissue Donors