Kinin-B2 Receptor Activity in Skeletal Muscle Regeneration and Myoblast Differentiation.

Abstract

The bioactive peptide bradykinin obtained from cleavage of precursor kininogens activates the kinin-B2 receptor functioning in induction of inflammation and vasodilatation. In addition, bradykinin participates in kidney and cardiovascular development and neuronal and muscle differentiation. Here we show that kinin-B2 receptors are expressed throughout differentiation of murine C2C12 myoblasts into myotubes. An autocrine loop between receptor activation and bradykinin secretion is suggested, since bradykinin secretion is significantly reduced in the presence of the kinin-B2 receptor antagonist HOE-140 during differentiation. Expression of skeletal muscle markers and regenerative capacity were decreased after pharmacological inhibition or genetic ablation of the B2 receptor, while its antagonism increased the number of myoblasts in culture. In summary, the present work reveals to date no functions described for the B2 receptor in muscle regeneration due to the control of proliferation and differentiation of muscle precursor cells.

• Authors: Janaina M Alves, Antonio H Martins, Claudiana Lameu, Talita Glaser, Nawal M Boukli, Vinicius Bassaneze, Rafael Dariolli, Isis C Nascimento, Poliana C M Martins, Héllio D N de Souza, José Eduardo Krieger, Dulce E Casarini, Vicencia M Sales, João B Pesquero, Henning Ulrich
• Journal: Stem cell reviews and reports (Stem Cell Rev Rep) Vol. 15 Issue 1 Pg. 48-58 (02 2019) ISSN: 2629-3277 [Electronic] United States
• PMID: 30338498 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Cardiotoxins
• Kininogens
• RNA, Messenger
• Receptor, Bradykinin B2
• Myosin Heavy Chains
• Bradykinin

Topics

• Animals
• Biomarkers (metabolism)
• Bradykinin (metabolism)
• Cardiotoxins (administration & dosage)
• Cell Differentiation
• Cell Line
• Cell Proliferation
• Cytoskeleton (metabolism)
• Gene Deletion
• Kininogens (genetics, metabolism)
• Mice, Inbred C57BL
• Muscle Fibers, Skeletal (cytology, metabolism)
• Muscle, Skeletal (physiology)
• Myoblasts (cytology)
• Myosin Heavy Chains (metabolism)
• RNA, Messenger (genetics, metabolism)
• Receptor, Bradykinin B2 (genetics, metabolism)
• Regeneration