Leptin Increases Particle-Induced Osteolysis in Female ob/ob Mice.

Abstract

Particles generated from wear of prosthesis joint bearing surfaces induce inflammation-mediated periprosthetic bone resorption (osteolysis). Morbidly obese leptin-deficient ob/ob mice are resistant to polyethylene particle-induced bone loss, suggesting that leptin, a hormone produced by adipocytes that circulates in concentrations proportional to total body adiposity, increases osteolysis. To confirm that particles induce less osteolysis in leptin-deficient mice after controlling for cold stress (room temperature)-induced bone loss, ob/ob mice on a C57BL/6 (B6) background and colony B6 wildtype (WT) mice housed at thermoneutral temperature were randomized to control or particle treatment groups (N = 5/group). Polyethylene particles were implanted over calvaria and mice sacrificed 2 weeks later. Compared to particle-treated WT mice, particle-treated ob/ob mice had lower osteolysis score, less infiltration of immune cells, and less woven bone formation. To determine the role of leptin in particle-induced osteolysis, ob/ob mice were randomized into one of 4 groups (n = 6-8/group): (1) control, (2) particles, (3) particles + continuous leptin (osmotic pump, 6 μg/d), or (4) particles + intermittent leptin (daily injection, 40 μg/d). Leptin treatment increased particle-induced osteolysis in ob/ob mice, providing evidence that the adpiokine may play a role in inflammation-driven bone loss. Additional research is required to determine whether altering leptin levels within the physiological range results in corresponding changes in polyethylene-particle-induced osteolysis.

• Authors: Kenneth A Philbrick, Adam J Branscum, Carmen P Wong, Russell T Turner, Urszula T Iwaniec
• Journal: Scientific reports (Sci Rep) Vol. 8 Issue 1 Pg. 14790 (10 04 2018) ISSN: 2045-2322 [Electronic] England
• PMID: 30287858 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Leptin

Topics

• Animals
• Female
• Leptin (deficiency, metabolism)
• Mice, Inbred C57BL
• Mice, Knockout
• Mice, Obese
• Osteoarthritis (pathology)
• Osteolysis
• Prostheses and Implants (adverse effects)