Abstract | BACKGROUND: METHODS: The analysis included plasma samples obtained from 36 patients diagnosed with MF in our center from 2004 to 2016 (JAK2 V617-positive 53%; CALR-positive 31%; MPL-positive 14%; triple negative 2%) and a control group comprising 20 healthy volunteer blood donors. Thrombin generation was measured in platelet-rich plasma using the TECHNOTHROMBIN® TGA kit (Technoclone, Austria) and the fully automated system Ceveron® Alpha (Technoclone). The results were correlated with clinical and laboratory parameters of the patients. RESULTS: There were differences in thrombin generation as expressed by endogenous thrombin potential (ETP) between patients and healthy controls, with ETP being lower in the patient group (p = 0.0003). Analysis confirmed a significant correlation between thrombin generation and platelet counts, with higher thrombin generation in patients with thrombocythemia > 400 x 109/L (p = 0.04). ETP values were consistently higher in earlier disease stages and lower in CALR-mutated myelofibrosis. CONCLUSIONS: In MF patients, thrombin generation is mainly influenced by platelet counts and, to a lesser extent, by mutation status, activity, and progression of the disease. Thrombin generation test results have confirmed that thrombocythemia is a potential risk factor for thrombotic complications.
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Authors | M Palova, L Slavik, A Hlusi, T Szotkowski, J Ulehlova, M Divoka, J Navratilova, T Papajik |
Journal | Clinical laboratory
(Clin Lab)
Vol. 64
Issue 9
Pg. 1373-1383
(Sep 01 2018)
ISSN: 1433-6510 [Print] Germany |
PMID | 30274004
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- CALR protein, human
- Calreticulin
- Receptors, Thrombopoietin
- MPL protein, human
- JAK2 protein, human
- Janus Kinase 2
- Thrombin
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Topics |
- Adult
- Aged
- Biomarkers
(blood)
- Blood Coagulation
- Blood Coagulation Tests
- Calreticulin
(genetics)
- Case-Control Studies
- Female
- Genetic Predisposition to Disease
- Humans
- Janus Kinase 2
(genetics)
- Male
- Middle Aged
- Mutation
- Phenotype
- Platelet Count
- Predictive Value of Tests
- Primary Myelofibrosis
(blood, complications, diagnosis, genetics)
- Prognosis
- Receptors, Thrombopoietin
(genetics)
- Risk Assessment
- Risk Factors
- Thrombin
(metabolism)
- Thrombosis
(blood, diagnosis, etiology)
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