Oxidative Stress-Protective and Anti-Melanogenic Effects of Loliolide and Ethanol Extract from Fresh Water Green Algae, Prasiola japonica.

Abstract

Loliolide is a monoterpenoid hydroxylactone found in many algae, including fresh water green algae, Prasiola japonica. To date, loliolide and compounds in P. japonica have not been studied systematically with respect to skin pharmacology. In this study, we investigated oxidative stress-protective and anti-melanogenic effects of loliolide and P. japonica ethanol extract (Pj-EE), known to contain loliolide, in human keratinocyte (HaCaT) cells and mouse melanoma (B16F10) cells. Loliolide suppressed the transcription of genes encoding matrix metalloproteinases (MMPS), which were induced in HaCaT cells by hydrogen peroxide (H₂O₂) treatment. Loliolide and Pj-EE not only reduced the melanin secretion and content in B16F10 cells but also increased the expression of the antioxidant proteins nuclear factor (erythroid-derived 2)-like 2 (NRF2) and heme oxygenase-1 (HO-1) in HaCaT cells subjected to H₂O₂ treatment. Furthermore, loliolide and Pj-EE decreased expression of the anti-melanogenic protein microphthalmia-associated transcription factor (MITF) and tyrosinase in B16F10 cells subjected to α-melanocyte-stimulating hormone (α-MSH) treatment. Our findings demonstrate that loliolide and Pj-EE have antioxidant and anti-melanogenic effects on skin.

• Authors: Sang Hee Park, Eunju Choi, Sunggyu Kim, Dong Sam Kim, Ji Hyeon Kim, SeokGu Chang, Jae Seok Choi, Kyung Ja Park, Kyung-Baeg Roh, Jongsung Lee, Byong Chul Yoo, Jae Youl Cho
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 19 Issue 9 (Sep 18 2018) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 30231594 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Antioxidants
• Benzofurans
• Melanins
• Microphthalmia-Associated Transcription Factor
• Monophenol Monooxygenase
• loliolide

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Antioxidants (chemistry, pharmacology)
• Benzofurans (chemistry, pharmacology)
• Cell Line
• Cell Line, Tumor
• Chlorophyta (chemistry)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Melanins (genetics, metabolism)
• Melanoma (drug therapy, genetics, metabolism)
• Mice
• Microphthalmia-Associated Transcription Factor (genetics, metabolism)
• Monophenol Monooxygenase (genetics, metabolism)
• Oxidative Stress (drug effects)