Abstract | BACKGROUND: Although assemblies of hydrophobic-modified bacitracin A with PLGA (Nano-BAPLGA) have demonstrated promising antibacterial activities against both Gram-positive and Gram-negative bacteria, the desirable antibacterial potency has remained challenging due to the low solubility of Nano-BAPLGA. To address this tissue, a series of Pluronic copolymers (Pluronic® F127, Pluronic® P123 and Pluronic® P85) were selected to link the N-terminus of bacitracin A to construct Pluronic-based nano-self assemblies (Nano-BAF127, Nano-BAP123 and Nano-BAP85). RESULTS: Impressively, all the newly designed Pluronic-based Nano-BAs possessed higher solubility and stronger effectiveness against both Gram-positive and Gram-negative bacteria compared with Nano-BAPLGA, especially the modification with Pluronic® P85. Surface tension measurements indicated that Nano-BAP85 was much more tensioactive than Nano-BAPLGA, which usually translated into a good membranolytic effect. Fluorescence spectroscopy and electron microscopy analyses confirmed the speculation that the cell wall/membrane might be the main action target of Nano-BAP85 by permeabilizing the cell membrane and damaging the membrane integrity. In vivo results further demonstrated that Nano-BAP85 significantly suppressed bacterial growth and prolonged survival time in the bacterial peritonitis mouse model with negligible toxicity. CONCLUSIONS:
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Authors | Wei Hong, Lipeng Liu, Yining Zhao, Yinghui Liu, Dexian Zhang, Mingchun Liu |
Journal | Journal of nanobiotechnology
(J Nanobiotechnology)
Vol. 16
Issue 1
Pg. 66
(Sep 11 2018)
ISSN: 1477-3155 [Electronic] England |
PMID | 30205822
(Publication Type: Journal Article, Retracted Publication)
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Chemical References |
- Anti-Bacterial Agents
- Micelles
- Poloxamer
- Bacitracin
- bacitracin A
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Topics |
- Animals
- Anti-Bacterial Agents
(chemistry, pharmacology, therapeutic use)
- Bacitracin
(chemistry, pharmacology, therapeutic use)
- Bacteria
(drug effects)
- Bacterial Infections
(drug therapy)
- Male
- Mice
- Micelles
- Peritonitis
(drug therapy)
- Poloxamer
(chemistry, pharmacology, therapeutic use)
- Solubility
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