The anti-anaphylactoid effects of hydroxysafflor yellow A on the suppression of mast cell Ca2+ influx and degranulation.

Abstract

BACKGROUND:

Anaphylaxis is a type of potentially fatal hypersensitivity reaction resulting from the activation of mast cell mediators, especially histamine and lipid mediators. Non-IgE-mediated anaphylaxis can occur because of the direct activation of mast cells. Hydroxysafflor yellow A (HSYA) is the main chemical component of safflower (Carthamus tinctorius) and has been reported to have pharmacological activities. However, the anti-anaphylactoid effect of HSYA has not yet been investigated.

PURPOSE:

The aims of this study were to evaluate the anti-anaphylactoid activity of HSYA in vivo and to investigate the underlying mechanism in vitro.

METHODS:

The anti-anaphylactoid activity of HSYA was evaluated in a mouse model of hindpaw extravasation. Calcium imaging was used to assess intracellular Ca2+ mobilization. The levels of cytokines and chemokines released by stimulated mast cells were measured using enzyme immunoassay kits. Western blotting was used to explore the related molecular signaling pathways.

RESULTS:

HSYA markedly inhibited mast cell degranulation by suppressing the activation of intracellular Ca2+ mobilization and preventing the release of cytokines and chemokines from mast cells in a dose-dependent manner via the PKC-PLCγ-IP3R signaling pathway.

CONCLUSION:

In summary, HSYA has anti-anaphylactoid pharmacological activity, which makes it a potential candidate for the development of a novel agent to suppress drug-induced anaphylactoid reactions.

• Authors: Rui Liu, Tingting Zhao, Delu Che, Jiao Cao, Jue Wang, Yanni Lv, Pengyu Ma, Yuanyuan Ding, Nana Wang, Xiaoyang Wang, Nan Wang, Jianli Wang, Zijun Gao, Tao Zhang
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 48 Pg. 43-50 (Sep 15 2018) ISSN: 1618-095X [Electronic] Germany
• PMID: 30195879 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier GmbH. All rights reserved.

Chemical References

• Cytokines
• Quinones
• hydroxysafflor yellow A
• Chalcone
• Calcium

Topics

• Anaphylaxis (drug therapy)
• Animals
• Calcium (metabolism)
• Carthamus tinctorius (chemistry)
• Cell Degranulation (drug effects)
• Cells, Cultured
• Chalcone (analogs & derivatives, pharmacology)
• Cytokines (metabolism)
• Disease Models, Animal
• Mast Cells (drug effects)
• Mice
• Mice, Inbred C57BL
• Quinones (pharmacology)
• Signal Transduction (drug effects)