MiR-320 regulates cardiomyocyte apoptosis induced by ischemia-reperfusion injury by targeting AKIP1.

Abstract

BACKGROUND:

MicroRNAs play important roles in regulation of the cardiovascular system. The purpose of this study was to investigate microRNA-320 (miR-320) expression in myocardial ischemia-reperfusion (I/R) injury and the roles of miR-320 in cardiomyocyte apoptosis by targeting AKIP1 (A kinase interacting protein 1).

METHODS:

The level of miR-320 was detected using quantitative real-time polymerase chain reaction (qRT-PCR), and cardiomyocyte apoptosis was detected via terminal dUTP nick end-labeling assay. Cardiomyocyte apoptosis and the mitochondrial membrane potential were evaluated via flow cytometry. Bioinformatics tools were used to identify the target gene of miR-320. The expression levels of AKIP1 mRNA and protein were detected via qRT-PCR and Western blot, respectively.

RESULTS:

Both the level of miR-320 and the rate of cardiomyocyte apoptosis were substantially higher in the I/R group and H9c2 cells subjected to H/R than in the corresponding controls. Overexpression of miR-320 significantly promoted cardiomyocyte apoptosis and increased the loss of the mitochondrial membrane potential, whereas downregulation of miR-320 had an opposite effect. Luciferase reporter assay showed that miR-320 directly targets AKIP1. Moreover, knock down and overexpression of AKIP1 had similar effects on the H9c2 cells subjected to H/R.

CONCLUSIONS:

miR-320 plays an important role in regulating cardiomyocyte apoptosis induced by I/R injury by targeting AKIP1 and inducing the mitochondrial apoptotic pathway.

• Authors: Zhi-Qiang Tian, Hong Jiang, Zhi-Bing Lu
• Journal: Cellular & molecular biology letters (Cell Mol Biol Lett) Vol. 23 Pg. 41 ( 2018) ISSN: 1689-1392 [Electronic] England
• PMID: 30181740 (Publication Type: Journal Article)

Chemical References

• AKIP1 protein, mouse
• AKIP1 protein, rat
• Adaptor Proteins, Signal Transducing
• MIRN320 microRNA, rat
• MicroRNAs
• Mirn320 microRNA, mouse

Topics

• Adaptor Proteins, Signal Transducing (genetics)
• Animals
• Apoptosis
• Cell Line
• Gene Expression Regulation
• Male
• Mice, Inbred C57BL
• MicroRNAs (genetics)
• Myocardial Reperfusion Injury (genetics, pathology)
• Myocytes, Cardiac (metabolism, pathology)
• Rats