The Thomsen-Friedenreich Antigen-Specific Antibody Signatures in Patients with Breast Cancer.

Abstract

Alterations in the glycosylation of serum total immunoglobulins show these antibodies to have a diagnostic potential for cancer but the disease-related Abs to the tumor-associated antigens, including glycans, have still poorly been investigated in this respect. We analysed serum samples from patients with breast carcinoma (n = 196) and controls (n = 64) for the level of Thomsen-Friedenreich (TF) antigen-specific antibody isotypes, their sialylation, interrelationships, and the avidity by using ELISA with the synthetic TF-polyacrylamide conjugate as an antigen and the sialic acid-specific Sambucus nigra agglutinin (SNA) and ammonium thiocyanate as a chaotrope. An increased sialylation of IgG and IgM, but a lower SNA reactivity of IgA TF antibodies, and a higher level and avidity of the TF-specific IgA were found in cancer patients. Other cancer-related signatures were the highly significant increase of the IgG/IgA ratio and the very low SNA/IgA index in cancer, including patients with an early stage of the disease. These changes showed a good diagnostic potential with about 80% accuracy. Thus, the level of naturally occurring anti-TF antigen antibodies, their sialylation profile, isotype distribution, and avidity displayed cancer-specific changes that could serve as novel noninvasive Ab-based biomarkers for early breast cancer.

• Authors: Oleg Kurtenkov, Kaire Innos, Boris Sergejev, Kersti Klaamas
• Journal: BioMed research international (Biomed Res Int) Vol. 2018 Pg. 9579828 ( 2018) ISSN: 2314-6141 [Electronic] United States
• PMID: 30105268 (Publication Type: Journal Article)

Chemical References

• Agglutinins
• Antigens, Tumor-Associated, Carbohydrate
• Thomsen-Friedenreich antigen

Topics

• Adult
• Aged
• Agglutinins
• Antigens, Tumor-Associated, Carbohydrate (analysis)
• Breast Neoplasms (diagnosis, immunology)
• Female
• Humans
• Middle Aged
• Neoplasm Staging
• Young Adult