[Interference of Lumican Regulates the Invasion and Migration of Liver Cancer Cells].

Abstract

OBJECTIVE:

To explore the effect of silencing lumican on the invasion and migration of liver cancer cells.

METHODS:

Lumican was silenced by shRNA in liver cancer cells (HepG2 and MHCC97H). The mRNA levels of lumican were detected by qRT-PCR. Cell invasion was measured by Transwell. Cell migration was tested via wound healing. The protein levels of lumican,MMP-9,VEGF,ERK1,JNK,p-ERK1 and p-JNK were measured by Western blot.

RESULTS:

Liver cancer cells (HepG2 and MHCC97H) had higher levels of mRNA and protein of lumican compared with normal hepatocyte L02 (P<0.01). shRNA lowered the levels of mRNA and protein of lumican (P<0.01),and weakened the invasion and migration of cancer cells (P<0.01). The expressions of MMP-9 and VEGF decreased with the shRNA silence (P<0.01). shRNA also reduced the protein level of p-ERK1 and p-JNK (P<0.01).

CONCLUSION:

Silencing lumican by shRNA attenuates the invasion and migration of liver cancer cells via inhibiting the activation of ERK1/JNK pathway.

• Authors: Qi-Ming Mu, Wei He, Gui-Min Hou, Yong Liang, Gang Wang, Chun-Lin Li, Bo Liao, Xin Liu, Zhi Ye, Jian-Li Lu, Wen-Tao Wang
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 49 Issue 3 Pg. 358-363 (May 2018) ISSN: 1672-173X [Print] China
• PMID: 30014634 (Publication Type: Journal Article)
• Copyright: Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).

Chemical References

• Lumican
• RNA, Small Interfering
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• Mitogen-Activated Protein Kinase 3
• MAP Kinase Kinase 4
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Cell Line, Tumor
• Cell Movement
• Gene Silencing
• Humans
• Liver Neoplasms (genetics, pathology)
• Lumican (genetics, metabolism)
• MAP Kinase Kinase 4 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Mitogen-Activated Protein Kinase 3 (metabolism)
• Neoplasm Invasiveness
• RNA, Small Interfering
• Vascular Endothelial Growth Factor A (metabolism)