OTULIN limits cell death and inflammation by deubiquitinating LUBAC.

Abstract

OTULIN (OTU deubiquitinase with linear linkage specificity) removes linear polyubiquitin from proteins that have been modified by LUBAC (linear ubiquitin chain assembly complex) and is critical for preventing auto-inflammatory disease1,2 and embryonic lethality during mouse development3. Here we show that OTULIN promotes rather than counteracts LUBAC activity by preventing its auto-ubiquitination with linear polyubiquitin. Thus, knock-in mice that express catalytically inactive OTULIN, either constitutively or selectively in endothelial cells, resembled LUBAC-deficient mice4 and died midgestation as a result of cell death mediated by TNFR1 (tumour necrosis factor receptor 1) and the kinase activity of RIPK1 (receptor-interacting protein kinase 1). Inactivation of OTULIN in adult mice also caused pro-inflammatory cell death. Accordingly, embryonic lethality and adult auto-inflammation were prevented by the combined loss of cell death mediators: caspase 8 for apoptosis and RIPK3 for necroptosis. Unexpectedly, OTULIN mutant mice that lacked caspase 8 and RIPK3 died in the perinatal period, exhibiting enhanced production of type I interferon that was dependent on RIPK1. Collectively, our results indicate that OTULIN and LUBAC function in a linear pathway, and highlight a previously unrecognized interaction between linear ubiquitination, regulators of cell death, and induction of type I interferon.

• Authors: Klaus Heger, Katherine E Wickliffe, Ada Ndoja, Juan Zhang, Aditya Murthy, Debra L Dugger, Allie Maltzman, Felipe de Sousa E Melo, Jeffrey Hung, Yi Zeng, Erik Verschueren, Donald S Kirkpatrick, Domagoj Vucic, Wyne P Lee, Merone Roose-Girma, Robert J Newman, Søren Warming, Yi-Chun Hsiao, László G Kőműves, Joshua D Webster, Kim Newton, Vishva M Dixit
• Journal: Nature (Nature) Vol. 559 Issue 7712 Pg. 120-124 (07 2018) ISSN: 1476-4687 [Electronic] England
• PMID: 29950720 (Publication Type: Journal Article)

Chemical References

• Interferon Type I
• Ubiquitin
• Receptor-Interacting Protein Serine-Threonine Kinases
• Ripk3 protein, mouse
• Endopeptidases
• gumby protein, mouse
• Deubiquitinating Enzymes
• Casp8 protein, mouse
• Caspase 8

Topics

• Animals
• Caspase 8 (genetics, metabolism)
• Cell Death (genetics)
• Deubiquitinating Enzymes (genetics, metabolism)
• Embryo Loss (genetics)
• Endopeptidases (genetics, metabolism)
• Inflammation (enzymology, genetics, metabolism)
• Interferon Type I (biosynthesis)
• Mice
• Mice, Inbred C57BL
• Receptor-Interacting Protein Serine-Threonine Kinases (deficiency, genetics, metabolism)
• Ubiquitin (chemistry, metabolism)
• Ubiquitination (genetics)
• Weight Loss (genetics)