Impaired Local Translation of β-actin mRNA in Ighmbp2-Deficient Motoneurons: Implications for Spinal Muscular Atrophy with respiratory Distress (SMARD1).

Abstract

Spinal muscular atrophy with respiratory distress type 1 (SMARD1) is a fatal motoneuron disorder in children with unknown etiology. The disease is caused by mutations in the IGHMBP2 gene, encoding a Super Family 1 (SF1)-type RNA/DNA helicase. IGHMBP2 is a cytosolic protein that binds to ribosomes and polysomes, suggesting a role in mRNA metabolism. Here we performed morphological and functional analyses of isolated immunoglobulin μ-binding protein 2 (Ighmbp2)-deficient motoneurons to address the question whether the SMARD1 phenotype results from de-regulation of protein biosynthesis. Ighmbp2-deficient motoneurons exhibited only moderate morphological aberrations such as a slight increase of axonal branches. Consistent with the rather mild phenotypic aberrations, RNA sequencing of Ighmbp2-deficient motoneurons revealed only minor transcriptome alterations compared to controls. Likewise, we did not detect any global changes in protein synthesis using pulsed SILAC (Stable Isotope Labeling by Amino acids in Cell culture), FUNCAT (FlUorescent Non-Canonical Amino acid Tagging) and SUnSET (SUrface SEnsing of Translation) approaches. However, we observed reduced β-actin protein levels at the growth cone of Ighmbp2-deficient motoneurons which was accompanied by reduced level of IMP1/ZBP1, a known interactor of β-actin mRNA. Fluorescence Recovery after Photobleaching (FRAP) studies revealed translational down-regulation of an eGFP-myr-β-actin 3'UTR mRNA in growth cones. Local translational regulation of β-actin mRNA was dependent on the 3' UTR but independent of direct Ighmbp2-binding to β-actin mRNA. Taken together, our data indicate that Ighmbp2 deficiency results in local but modest disruption of protein biosynthesis which might partially contribute to the motoneuron defects seen in SMARD1.

• Authors: Verena Surrey, Caren Zöller, Alicia Andrea Lork, Mehri Moradi, Stefanie Balk, Benjamin Dombert, Lena Saal-Bauernschubert, Michael Briese, Silke Appenzeller, Utz Fischer, Sibylle Jablonka
• Journal: Neuroscience (Neuroscience) Vol. 386 Pg. 24-40 (08 21 2018) ISSN: 1873-7544 [Electronic] United States
• PMID: 29928949 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 IBRO. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Actins
• DNA-Binding Proteins
• Ighmbp2 protein, mouse
• RNA, Messenger
• Transcription Factors

Topics

• Actins (genetics)
• Animals
• Cells, Cultured
• DNA-Binding Proteins (deficiency, genetics)
• Female
• HEK293 Cells
• Humans
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Motor Neurons (metabolism, pathology)
• Muscular Atrophy, Spinal (genetics, pathology)
• Pregnancy
• Protein Biosynthesis (genetics)
• RNA, Messenger (genetics)
• Respiratory Distress Syndrome, Newborn (genetics, pathology)
• Transcription Factors (deficiency, genetics)