Prospects of developing a prophylactic vaccine against human lymphatic filariasis - evaluation of protection in non-human primates.

Abstract

Lymphatic filariasis (LF) affects 120 million people around the world and another 856 million people are at risk of acquiring the infection. Mass Drug Administration (MDA) spearheaded by the World Health Organization is the only current strategy to control this infection. Recent reports suggest that despite several rounds of MDA, elimination has not been achieved and there is a need for more stringent control strategies for control of LF. An effective prophylactic vaccine combined with MDA has significant potential. Initial trials using a prophylactic trivalent recombinant Brugia malayi heat shock protein 12.6, abundant larval transcript -2 and tetraspanin large extra-cellular loop (rBmHAT) vaccine developed in our laboratory conferred only 35% protection in macaques. Therefore, the focus of the present study was to improve the current vaccine formulation to obtain better protection in non-human primates. We made two modifications to the current formulation: (i) the addition of another antigen, thioredoxin peroxidase-2 (TPX-2) to make it a tetravalent vaccine (rBmHAXT) and (ii) the inclusion of an adjuvant; AL019 (alum plus glucopyranosyl lipid adjuvant-stable emulsion) that is known to promote a balanced Th1/Th2 response. A double-blinded vaccination trial was performed with 40 macaques that were divided into three treatment groups and one control group (n = 10/group). Vaccinated animals received 4 immunisations at 1 month intervals with 150 µg/ml of rBmHAT plus alum, rBmHAT plus AL019 or rBmHAXT plus AL019. Control animals received AL019 only. All vaccinated macaques developed significant (P ≤ 0.003) titers of antigen-specific IgG antibodies (1:20,000) compared with the controls. One month after the last dose, all macaques were challenged s.c. with 130-180 B. malayi L3s. Our results showed that seven out of 10 (70%) of macaques given the improved rBmHAXT vaccine did not develop the infection compared with AL019 controls, of which seven out of 10 macaques developed the infection. Titers of antigen-specific IgG1 and IgG2 antibodies were significantly (P ≤ 0.01) higher in vaccinated animals and there was an increase in the percentage of IL-4 and IFN-γ secreting antigen-responding memory T cells. These studies demonstrated that the improved formulation (rBmHAXT plus AL019) is a promising vaccine candidate against human lymphatic filariasis.

• Authors: Vishal Khatri, Nikhil Chauhan, Kanchan Vishnoi, Agneta von Gegerfelt, Courtney Gittens, Ramaswamy Kalyanasundaram
• Journal: International journal for parasitology (Int J Parasitol) Vol. 48 Issue 9-10 Pg. 773-783 (08 2018) ISSN: 1879-0135 [Electronic] England
• PMID: 29885437 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2018 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Helminth
• Antigens, Helminth
• Helminth Proteins
• Immunoglobulin G
• Recombinant Proteins
• Vaccines
• Interleukin-4
• Interferon-gamma

Topics

• Animals
• Antibodies, Helminth (blood)
• Antigens, Helminth (immunology)
• Brugia malayi (immunology)
• Cloning, Molecular
• Elephantiasis, Filarial (prevention & control)
• Escherichia coli
• Female
• Filariasis (prevention & control)
• Gene Expression Regulation
• Helminth Proteins (genetics, immunology)
• Humans
• Immunoglobulin G (classification, physiology)
• Interferon-gamma (metabolism)
• Interleukin-4 (metabolism)
• Macaca mulatta
• Male
• Recombinant Proteins (immunology, metabolism)
• Vaccines (immunology)