The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes.

Abstract

Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.

• Authors: Yu-Chu Wang, Kung-Chao Chang, Bo-Wen Lin, Jenq-Chang Lee, Chien-Hsien Lai, Li-Jyuan Lin, Yun Yen, Chang-Shen Lin, Shiang-Jie Yang, Peng-Chan Lin, Chung-Ta Lee, Liang-Yi Hung
• Journal: Experimental & molecular medicine (Exp Mol Med) Vol. 50 Issue 6 Pg. 1-14 (06 06 2018) ISSN: 2092-6413 [Electronic] United States
• PMID: 29884818 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Heterogeneous-Nuclear Ribonucleoproteins
• Neoplasm Proteins
• SYNCRIP protein, human
• Epidermal Growth Factor

Topics

• Cell Transformation, Neoplastic (genetics, metabolism, pathology)
• Colorectal Neoplasms (genetics, metabolism, pathology)
• Epidermal Growth Factor (genetics, metabolism)
• HCT116 Cells
• Heterogeneous-Nuclear Ribonucleoproteins (genetics, metabolism)
• Humans
• MAP Kinase Signaling System
• Mitosis
• Neoplasm Proteins (genetics, metabolism)