Abstract | OBJECTIVES: METHODS: RBC (n = 14) were washed/resuspended in NS or PL-A. Free hemoglobin and heme were determined at 0, 24, 48, and 72 hours. Platelet concentrates (PCs; n = 21) were washed with NS or PL-A and resuspended in same washing solution (n = 13) or ABO-identical plasma (n = 8). Platelet aggregation and spreading were evaluated. RESULTS: The 24-hour free hemoglobin and heme levels were higher in NS (P < .05). Improved platelet function was observed in PL-A-washed PCs (P < .001). DISCUSSION: PL-A showed less RBC hemolysis and better platelet function than NS. Whether such differences would occur in vivo is unknown.
|
Authors | Majed A Refaai, Grace W Conley, Kelly F Henrichs, Hannah McRae, Amy E Schmidt, Richard P Phipps, Sherry L Spinelli, Debra Masel, Jill M Cholette, Anthony Pietropaoli, Michael P Eaton, Neil Blumberg |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 150
Issue 2
Pg. 146-153
(Jul 03 2018)
ISSN: 1943-7722 [Electronic] England |
PMID | 29878038
(Publication Type: Journal Article)
|
Chemical References |
- Electrolytes
- Saline Solution
- Plasmalyte A
|
Topics |
- Blood Platelets
- Blood Specimen Collection
(methods)
- Electrolytes
- Erythrocytes
- Hemolysis
- Humans
- Saline Solution
- Transfusion Reaction
(prevention & control)
|