Toxoplasma gondii GRA8 induces ATP5A1-SIRT3-mediated mitochondrial metabolic resuscitation: a potential therapy for sepsis.

Abstract

The intracellular parasite Toxoplasma gondii has unique dense granule antigens (GRAs) that are crucial for host infection. Emerging evidence suggests that GRA8 of T. gondii is a promising serodiagnostic marker in toxoplasmosis. However, little is known about the intracellular regulatory mechanisms involved in GRA8-induced host responses. We found that GRA8 interacts with host proteins involved in mitochondria activation and might be useful as a therapeutic strategy for sepsis. Here, we show that protein kinase-Cα (PKCα)-mediated phosphorylation of T. gondii GRA8 (Thr220) is required for mitochondrial trafficking and regulates the interaction of C terminal of GRA8 with nucleotide binding domain of ATP5A1. Furthermore, GRA8 interacts with SIRT3 in mitochondria, facilitating ATP5A1 deacetylation (K506 and K531), adenosine triphosphate production and subsequent anti-septic activity in vivo. Taken together, these results demonstrate a new anti-sepsis therapeutic strategy using T. gondii GRA8-induced mitochondrial metabolic resuscitation. This strategy represents an urgently needed paradigm shift for therapeutic intervention.

• Authors: Ye-Ram Kim, Jae-Sung Kim, Jin-Seung Yun, Sojin Kim, Sun Young Kim, Kiseok Jang, Chul-Su Yang
• Journal: Experimental & molecular medicine (Exp Mol Med) Vol. 50 Issue 3 Pg. e464 (03 30 2018) ISSN: 2092-6413 [Electronic] United States
• PMID: 29869623 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Protozoan
• GRA8 protein, Toxoplasma gondii
• Protozoan Proteins
• Sirtuin 3
• Mitochondrial Proton-Translocating ATPases

Topics

• Animals
• Antigens, Protozoan (genetics, metabolism)
• Female
• HEK293 Cells
• Humans
• Mass Spectrometry
• Mice
• Mice, Inbred C57BL
• Mitochondria (metabolism)
• Mitochondrial Proton-Translocating ATPases (genetics, metabolism)
• Phosphorylation
• Protein Binding
• Protozoan Proteins (genetics, metabolism)
• Sepsis (metabolism)
• Sirtuin 3 (genetics, metabolism)
• Toxoplasmosis (metabolism)