Panax ginseng is well known for its medicinal functions. As a class of important compound of ginseng,
ginsenoside is widely studied around the world. In addition, ginseng
glycopeptides also showed good
biological activity, but researches in this field are rarely reported. In this study, ginseng
glycopeptides (Gg) were first prepared from Panax ginseng by reflux extracted with 85%
ethanol and the following purification with
Sephadex G-15 column. Then, the inflammatory
pain models induced by
carrageenan and the rat
pain models induced by Faure Marin were established for research on mechanism of
analgesic activities. It is showed that Gg had an obvious inhibiting effect on
inflammation and a significant reduction on the
Malondialdehyde (MDA) of inflammatory foot tissue. And there were significant differences between moderate to high dose of Gg and model group in
Interleukin 1β (IL-1β),
Interleukin 2 (IL-2),
Interleukin 4 (IL-4),
Tumor necrosis factor α (TNF-α) and
Histamine. The two models can be preliminarily determined that the
analgesic effect of Gg may be peripheral, which mechanism may be related to the dynamic balance between proinflammatory
cytokines (TNF-α, IL-1β) and anti-inflammatory
cytokines (IL-2, IL-4, and
Interleukin 10 (IL-10)). A series of methods were used to study Gg in physical-chemical properties and linking mode of
glycoside. The high-resolution mass spectrometry was used for identification of the structure of Gg. Moreover, the structure of 20 major Gg were investigated and identified. The structural analysis of Gg was benefit for the next study on structure-activity relationship.