When
brain injury happens, endogenous neural stem cells (NSCs) located in the adult subventricular zone (SVZ) and subgranular zone (SGZ) are attacked by
ischemia/reperfusion to undergo cellular apoptosis and death before being induced to migrate to the lesion point and differentiate into mature neural cells for damaged cell replacement. Although promoting antiapoptosis and NSC survival are critical to neuroregeneration, the mechanism has yet been elucidated clearly. Here in this study, we established an in vitro
oxygen-
glucose deprivation (OGD)/reoxygenation model on NSCs and detected
glucose-regulated
protein 78 (
GRP78) involved in apoptosis, while in the absence of
GRP78 by
siRNA transfection, OGD/reoxygenation triggered PI3K/Akt, ERK1/2, and NF-κB/p65 activation, and induced NSC apoptosis was attenuated. Further investigation, respectively, with the inhibitor of PI3K/Akt or ERK1/2 demonstrated a blockage on
GRP78 upregulation, while the inhibition of NF-κB rarely affected
GRP78 induction by OGD/reoxygenation. The results indicated the bidirectional regulations of
GRP78-PI3K/Akt and
GRP78-ERK1/2 and the one-way signalling transduction through
GRP78 to NF-κB/p65 on NSC survival from OGD/reoxygenation. In conclusion, we found that
GRP78 mediated the signalling cross talk through PI3K/Akt, ERK1/2, and NF-κB/p65, which leads to antiapoptosis and NSC survival from
ischemic stroke. Our finding gives a new evidence of
GRP78 in NSCs as well as a new piece of signalling mechanism elucidation to NSC survival from
ischemic stroke.