Multiplatform profiling of meningioma provides molecular insight and prioritization of drug targets for rational clinical trial design.

Abstract

INTRODUCTION:

Surgery and radiation therapy are the standard treatment options for meningiomas, but these treatments are not always feasible. Expression profiling was performed to determine the presence of therapeutic actionable biomarkers for prioritization and selection of agents.

METHODS:

Meningiomas (n = 115) were profiled using a variety of strategies including next-generation sequencing (592-gene panel: n = 14; 47-gene panel: n = 94), immunohistochemistry (n = 8-110), and fluorescent and chromogenic in situ hybridization (n = 5-70) to determine mutational and expression status.

RESULTS:

The median age of patients in the cohort was 60 years, with a range spanning 6-90 years; 52% were female. The most frequently expressed protein markers were EGFR (93%; n = 44), followed by PTEN (77%; n = 110), BCRP (75%; n = 8), MRP1 (65%, n = 23), PGP (62%; n = 84), and MGMT (55%; n = 97). The most frequent mutation among all meningioma grades occurred in the NF2 gene at 85% (11/13). Recurring mutations in SMO and AKT1 were also occasionally detected. PD-L1 was expressed in 25% of grade III cases (2/8) but not in grade I or II tumors. PD-1 + T cells were present in 46% (24/52) of meningiomas. TOP2A and thymidylate synthase expression increased with grade (I = 5%, II = 22%, III = 62% and I = 5%, II = 23%, III = 47%, respectively), whereas progesterone receptor expression decreased with grade (I = 79%, II = 41%, III = 29%).

CONCLUSION:

If predicated on tumor expression, our data suggest that therapeutics directed toward NF2 and TOP2A could be considered for most meningioma patients.

• Authors: Richard G Everson, Yuuri Hashimoto, Jacob L Freeman, Tiffany R Hodges, Jason Huse, Shouhao Zhou, Joanne Xiu, David Spetzler, Nader Sanai, Lyndon Kim, Santosh Kesari, Andrew Brenner, Franco De Monte, Amy Heimberger, Shaan M Raza
• Journal: Journal of neuro-oncology (J Neurooncol) Vol. 139 Issue 2 Pg. 469-478 (Sep 2018) ISSN: 1573-7373 [Electronic] United States
• PMID: 29846894 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (genetics, metabolism)
• Child
• Clinical Trials as Topic (methods)
• Cohort Studies
• Female
• Gene Expression Regulation, Neoplastic
• High-Throughput Nucleotide Sequencing
• Humans
• Immunohistochemistry
• In Situ Hybridization
• Male
• Meningeal Neoplasms (drug therapy, genetics, metabolism, pathology)
• Meningioma (drug therapy, genetics, metabolism, pathology)
• Middle Aged
• Mutation
• Neoplasm Grading
• Research Design
• Young Adult