Development of human-derived hemoglobin-albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification.

Abstract

Novel hemoglobin-based artificial oxygen carriers are necessary in tissue engineering. We developed human hemoglobin (hHb) and albumin (HSA)-based microspheres using Shirasu porous glass (SPG) membrane emulsification. The obtained microspheres had a uniform size with an average diameter of 15.1 μm measured by optical microscope, which is similar to the diameter of human red blood cells (7-8 μm). The loading amount of hHb in the microspheres was 20 wt%, which is similar to that of red blood cells (33 wt%). The hHb-HSA microspheres showed similar oxygen dissociation behavior and methemoglobin formation resistance to native hHb. Incubation with genetically engineered HeLa cells that shows hypoxia-responsive EGFP expression demonstrated efficient oxygen supply from the microspheres. Our study suggests the utility of hHb-HSA microspheres as oxygen carriers for tissue engineering with a low risk of infectious disease.

• Authors: Seiichi Ohta, Kenichiro Hashimoto, Xiaoting Fu, Masamichi Kamihira, Yasuyuki Sakai, Taichi Ito
• Journal: Journal of bioscience and bioengineering (J Biosci Bioeng) Vol. 126 Issue 4 Pg. 533-539 (Oct 2018) ISSN: 1347-4421 [Electronic] Japan
• PMID: 29776730 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Chemical References

• Albumins
• Emulsions
• Hemoglobins
• Membranes, Artificial
• Oxygen

Topics

• Albumins (chemistry, metabolism)
• Emulsions (chemistry, metabolism)
• Erythrocytes (chemistry, metabolism)
• Glass (chemistry)
• HeLa Cells
• Hemoglobins (chemistry, metabolism)
• Humans
• Membranes
• Membranes, Artificial
• Microspheres
• Oxygen (metabolism)
• Particle Size
• Porosity