A novel plasma circular RNA circFARSA is a potential biomarker for non-small cell lung cancer.
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| Abstract |
Emerging evidence indicates that circular RNAs (circRNAs) are implicated in cancer development. This study aimed to evaluate whether circulating circRNAs may serve as novel biomarkers for non-small cell lung cancer (NSCLC). We used RNA sequencing (RNA-seq) and quantitative real-time PCR to explore cancer-related circRNAs. Bioinformatics and functional analyses were performed to reveal biological effects of circRNAs on lung cancer cells. A total of 5471 distinct circRNAs were identified by total RNA-seq, in which 185 were differentially expressed between cancerous and adjacent normal tissues. A circRNA derived from exon 5-7 of the FARSA gene, termed circFARSA, was observed to increase in cancerous tissues (P = 0.016), and was more abundant in patients' plasma than controls (P < 0.001). Overexpression of circFARSA in A549 cell line significantly promoted cell migration and invasion. In silico analysis suggested that circFARSA might sponge miR-330-5p and miR-326, thereby relieving their inhibitory effects on oncogene fatty acid synthase. Summarily, this study revealed circRNA profile of NSCLC for the first time and provided the evidence of plasma circFARSA as a potential noninvasive biomarker for this malignancy.
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| Authors | Dong Hang, Jing Zhou, Na Qin, Wen Zhou, Hongxia Ma, Guangfu Jin, Zhibin Hu, Juncheng Dai, Hongbing Shen |
| Journal | Cancer medicine (Cancer Med) Vol. 7 Issue 6 Pg. 2783-2791 (06 2018) ISSN: 2045-7634 [Electronic] United States |
| PMID | 29722168 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. |
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