The Anti-Periodontitis Effects of Ethanol Extract Prepared Using Lactobacillus paracasei subsp. paracasei NTU 101.

Abstract

Poor oral health and related diseases, including caries, periodontal disease, and oral cancer, are highly prevalent across the world, particularly in the elderly. This study aimed to investigate the anti-periodontitis activity of fermented skim milk produced using the promising probiotic Lactobacillus paracasei subsp. paracasei NTU 101 (NTU101FM). An initial analysis found that an ethanol extract of NTU101FM displayed anti-oxidative activities. Further investigation of pathogen growth inhibition zones, minimum inhibitory concentrations (MICs), and minimum bactericidal concentrations (MBCs) revealed that the NTU101FM ethanol extract also had anti-periodontal pathogen activities. In addition, the NTU101FM ethanol extract significantly decreased the release of pro-inflammatory cytokines induced by lipopolysaccharide (LPS) in RAW 264.7 macrophage cells. Finally, the NTU101FM ethanol extract was found to inhibit receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation by reducing tartrate-resistant acid phosphatase (TRAP) activity and the number of TRAP-positive multinucleated osteoclasts. In summary, our study demonstrated that ethanol extract prepared from NTU101FM has potential use as an anti-periodontitis agent.

• Authors: Te-Hua Liu, Tsung-Yu Tsai, Tzu-Ming Pan
• Journal: Nutrients (Nutrients) Vol. 10 Issue 4 (Apr 12 2018) ISSN: 2072-6643 [Electronic] Switzerland
• PMID: 29649103 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Anti-Inflammatory Agents
• Antioxidants
• Cytokines
• Inflammation Mediators
• RANK Ligand
• Solvents
• Tnfsf11 protein, mouse
• Ethanol
• Tartrate-Resistant Acid Phosphatase

Topics

• Aggregatibacter actinomycetemcomitans (drug effects, growth & development)
• Animals
• Anti-Bacterial Agents (isolation & purification, pharmacology)
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Antioxidants (isolation & purification, pharmacology)
• Bone Resorption (metabolism, prevention & control)
• Cultured Milk Products (microbiology)
• Cytokines (metabolism)
• Dose-Response Relationship, Drug
• Ethanol (chemistry)
• Inflammation Mediators (metabolism)
• Lacticaseibacillus paracasei (physiology)
• Macrophages (drug effects, metabolism)
• Mice
• Osteoclasts (drug effects, metabolism)
• Osteogenesis (drug effects)
• Oxidative Stress (drug effects)
• Periodontitis (metabolism, microbiology, prevention & control)
• Porphyromonas gingivalis (drug effects, growth & development)
• RANK Ligand (metabolism)
• RAW 264.7 Cells
• Solvents (metabolism)
• Tartrate-Resistant Acid Phosphatase (metabolism)