The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo.

Abstract

OBJECTIVE:

The objective of the study was to explore the role of calgranulin B gene on the biological behavior of squamous cervical cancer.

METHODS:

Differential transcription in calgranulin B gene between human papillomavirus (HPV)-positive and negative cervical cancer groups was identified, and the relationship between calgranulin B gene and matrix metalloproteinase (MMP) genes were explored using The Cancer Genome Atlas database. Subsequently, the role of calgranulin B on the cell proliferation, apoptosis, invasion and migration was investigated, through overexpression and/or underexpression of calgranulin B in cervical cancer cells. In addition, the effect of calgranulin B on the growth of the cervical cancer was studied via constructing xenograft model in BALB/c nude mice that either overexpressed or underexpressed calgranulin B.

RESULTS:

Calgranulin B gene transcription in cervical cancer was highly correlated with the high-risk HPV-16 and HPV-45. In addition, overexpression of calgranulin B increased cell proliferation, invasion and migration, whereas it did not significantly affect cell apoptosis. This effect was also confirmed by calgranulin B knockdown assay. Additionally, we found that the transcription of calgranulin B gene was negatively correlated with MMP15 and MMP24 genes, but positively associated with MMP25 genes in cervical cancer. Furthermore, calgranulin B significantly promoted the growth of cervical cancer in vivo.

CONCLUSION:

Calgranulin B promotes cell proliferation, migration and invasion of squamous cervical cancer, possibly via regulation of MMPs. Whether there are synergistic actions between calgranulin B and HPV-16/HPV-45 infection on the squamous cervical carcinogenesis or progression need further study.

• Authors: Wenwen Zhang, Miaomiao Chen, Huihui Cheng, Qi Shen, Ying Wang, Xueqiong Zhu
• Journal: Cancer management and research (Cancer Manag Res) Vol. 10 Pg. 323-338 ( 2018) ISSN: 1179-1322 [Print] New Zealand
• PMID: 29497331 (Publication Type: Journal Article, Retracted Publication)