To study the regulatory effect of
lncRNA HOTAIR/miR-20a-5p/HMGA2 axis on
breast cancer (BC) cell growth, cell mobility, invasiveness, and apoptosis. The microarray data of lncRNAs and mRNAs with differential expression in BC tissues were analyzed in the
Cancer Genome Atlas (TCGA) database.
LncRNA HOX transcript
antisense RNA (
lncRNA HOTAIR) expression in BC was assessed by qRT-PCR. Cell viability was confirmed using MTT and colony formation assay. Cell apoptosis was analyzed by TdT-mediated dUTP nick-end labeling (TUNEL) assay. Cell mobility and invasiveness were testified by transwell assay.
RNA pull-down and dual
luciferase assay were used for analysis of the correlation between
lncRNA HOTAIR and miR-20a-5p, as well as relationship of miR-20a-5p with high mobility group AT-hook 2 (HMGA2).
Tumor xenograft study was applied to confirm the correlation of
lncRNA HOTAIR/miR-20a-5p/HMGA2 axis on BC development in vivo. The expression levels of the
lncRNA HOTAIR were upregulated in BC tissues and cells. Knockdown
lncRNA HOTAIR inhibited cell propagation and
metastasis and facilitated cell apoptosis. MiR-20a-5p was a target of
lncRNA HOTAIR and had a negative correlation with
lncRNA HOTAIR. MiR-20a-5p overexpression in BC suppressed cell growth, mobility, and invasiveness and facilitated apoptosis. HMGA2 was a target of miR-20a-5p, which significantly induced
carcinogenesis of BC. BC cells progression was mediated by
lncRNA HOTAIR via affecting miR-20a-5p/HMGA2 in vivo.
LncRNA HOTAIR affected cell growth,
metastasis, and apoptosis via the miR-20a-5p/HMGA2 axis in
breast cancer.