Inhibition of STAT6/Anoctamin-1 Activation Suppresses Proliferation and Invasion of Gastric Cancer Cells.

Abstract

BACKGROUND:

Gastric carcinoma is the most popular cancer worldwide. Anoctamin-1 is a calcium-activated channel and highly expressed in various tumors. A previous study indicated that suppressed Anoctamin-1 expression decreased cancer cell proliferation or migration. As a signal transduction and transcription activator, STAT6 is a novel agonist for Anoctamin-1 promoter. However, its role in tumor cell proliferation or migration remains unclear. Therefore, this study aimed to suppress STAT6 and Anoctamin-1 protein expression in gastric cancer cells to test the inhibitory effects on gastric cancer cell migration or invasion.

MATERIALS AND METHODS:

MTT colorimetry was used to test cell proliferation. Western blot was used to measure STAT6 and Anoctamin-1 expression before and after small interfering RNA (siRNA) treatment. A scratch assay was performed to measure cell migration, followed by Transwell chamber assay analysis of cell invasion.

RESULTS:

After STAT6 siRNA interference, the expression of STAT6 and Anoctamin-1 was significantly decreased in the gastric carcinoma cell line. Anoctamin-1 siRNA interference only decreased its protein expression, but not STAT6 protein expression. Interference of STAT6 or Anoctamin-1 reduced their protein expression and inhibited proliferation, migration, or invasion of gastric cancer cells.

CONCLUSIONS:

Inhibition of STAT6/Anoctamin-1 activation decreased proliferation, migration, or invasion of gastric cancer cells, suggesting that the STAT6/Anoctamin-1 pathway might be a novel target for treating gastric cancer.

• Authors: Guohao Lu, Wanling Shi, Hongyu Zheng
• Journal: Cancer biotherapy & radiopharmaceuticals (Cancer Biother Radiopharm) Vol. 33 Issue 1 Pg. 3-7 (Feb 2018) ISSN: 1557-8852 [Electronic] United States
• PMID: 29466035 (Publication Type: Journal Article)

Chemical References

• ANO1 protein, human
• Anoctamin-1
• Neoplasm Proteins
• RNA, Small Interfering
• STAT6 Transcription Factor
• STAT6 protein, human

Topics

• Anoctamin-1 (antagonists & inhibitors, biosynthesis, genetics)
• Cell Line, Tumor
• Cell Movement (physiology)
• Cell Proliferation (physiology)
• Gene Knockdown Techniques
• Humans
• Neoplasm Invasiveness
• Neoplasm Proteins (antagonists & inhibitors, biosynthesis, genetics)
• RNA, Small Interfering (administration & dosage, genetics)
• STAT6 Transcription Factor (antagonists & inhibitors, biosynthesis, genetics)
• Stomach Neoplasms (genetics, metabolism, pathology)
• Transfection