All-in-One Theranostic Nanoplatform Based on Hollow MoSx for Photothermally-maneuvered Oxygen Self-enriched Photodynamic Therapy.

Abstract

Photodynamic therapy (PDT) kills cancer cells by converting tumor-dissolved oxygen into reactive singlet oxygen (1O2) using a photosensitizer under laser irradiation. However, pre-existing hypoxia in tumors and oxygen consumption during PDT can result in an inadequate oxygen supply, which in turn hampers PDT efficacy. Herein, an O2 self-sufficient nanotheranostic platform based on hollow MoSx nanoparticles (HMoSx) with oxygen-saturated perfluorohexane (O2@PFH) and surface-modified human serum albumin (HSA)/chloride aluminium phthalocyanine (AlPc) (O2@PFH@HMoSx-HSA/AlPc), has been designed for the imaging and oxygen self-enriched photodynamic therapy (Oxy-PDT) of cancer.

METHODS:

The in vitro anti-cancer activity and intracellular 1O2 generation performance of the nanoparticles were examined using 4T1 cells. We also evaluated the multimodal imaging capabilities and anti-tumor efficiency of the prepared nanoparticles in vivo using a 4T1 tumor-bearing nude mouse model.

RESULTS:

This nanoplatform could achieve the distinct in vivo fluorescence (FL)/photoacoustic (PA)/X-ray computed tomography (CT) triple-model imaging-guided photothermally-maneuvered Oxy-PDT. Interestingly, the fluorescence and Oxy-PDT properties of O2@PFH@HMoSx-HSA/AlPc were considerably quenched; however, photothermal activation by 670 nm laser irradiation induced a significant increase in temperature, which empowered the Oxy-PDT effect of the nanoparticles. In this study, O2@PFH@HMoSx-HSA/AlPc demonstrated a great potential to image and treat tumors both in vitro and in vivo, showing complete tumor-inhibition over 16 days after treatment in the 4T1 tumor model.

CONCLUSION:

O2@PFH@HMoSx-HSA/AlPc is promising to be used as novel multifunctional theranostic nanoagent for triple-modal imaging as well as single wavelength NIR laser triggered PTT/Oxy-PDT synergistic therapy.

• Authors: Jinping Wang, Li Liu, Qing You, Yilin Song, Qi Sun, Yidan Wang, Yu Cheng, Fengping Tan, Nan Li
• Journal: Theranostics (Theranostics) Vol. 8 Issue 4 Pg. 955-971 ( 2018) ISSN: 1838-7640 [Electronic] Australia
• PMID: 29463993 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Disulfides
• Fluorocarbons
• Oxidants
• Photosensitizing Agents
• Molybdenum
• perflexane
• molybdenum disulfide

Topics

• Animals
• Cell Line, Tumor
• Disulfides (metabolism)
• Fluorocarbons (metabolism)
• Hyperthermia, Induced (methods)
• Mammary Neoplasms, Experimental (diagnostic imaging, drug therapy)
• Mice, Nude
• Molybdenum (metabolism)
• Oxidants (metabolism)
• Photoacoustic Techniques
• Photochemotherapy (methods)
• Photosensitizing Agents (metabolism)
• Theranostic Nanomedicine (methods)
• Tomography, X-Ray Computed