Abstract |
Biological (or cellular) noise is the random quantitative variability of proteins and other molecules in individual, genetically identical cells. As the result of biological noise in the levels of some transcription factors that determine a cell's differentiation status, differentiated cells may dedifferentiate to a stem cell state given a sufficiently long time period. Here, to provide direct evidence supporting this hypothesis, we used a live-cell monitoring system based on enhanced green fluorescent protein (eGFP) expression to continuously assess the "stemness" of individual human and murine malignant mesothelioma cells over a period of up to 3 months. Re-expression of the transcription factors, the top hierarchical stemness markers Sox2 (SRY-box 2) and Oct4 (octamer-binding transcription factor), monitored as cell eGFP expression was observed in a subpopulation of differentiated eGFP(-) malignant mesothelioma cells. However, we found that this transition was extremely rare. Of note, when it did occur, neighboring cells that were not direct descendants of a newly emerged eGFP(+) stem cell were more likely than non-neighboring cells to also become an eGFP(+) stem cell. This observation suggested a positional effect and led to a clustered "mosaic" reappearance of eGFP(+) stem cells. Moreover, stem cells reappeared even in cell cultures derived from one single differentiated eGFP(-) cell. On the basis of our experimental in vitro and in vivo findings, we developed a tumor growth model to predict the clustered localization of cancer stem cells within a tumor mass.
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Authors | Walter Blum, Thomas Henzi, Beat Schwaller, László Pecze |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 293
Issue 14
Pg. 5247-5258
(04 06 2018)
ISSN: 1083-351X [Electronic] United States |
PMID | 29440274
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2018 by The American Society for Biochemistry and Molecular Biology, Inc. |
Chemical References |
- Biological Products
- Octamer Transcription Factor-3
- POU5F1 protein, human
- SOX2 protein, human
- SOXB1 Transcription Factors
- Transcription Factors
- enhanced green fluorescent protein
- Green Fluorescent Proteins
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Topics |
- Animals
- Artifacts
- Biological Products
(metabolism)
- Cell Culture Techniques
- Cell Differentiation
(physiology)
- Genes, Reporter
- Green Fluorescent Proteins
- Humans
- Mice
- Models, Biological
- Neoplastic Stem Cells
(metabolism, pathology)
- Octamer Transcription Factor-3
(metabolism)
- SOXB1 Transcription Factors
(metabolism)
- Transcription Factors
(metabolism)
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