Crambescidin 800, Isolated from the Marine Sponge Monanchora viridis, Induces Cell Cycle Arrest and Apoptosis in Triple-Negative Breast Cancer Cells.

Abstract

Triple negative breast cancer (TNBC) is currently the only group of breast cancers without an effective targeted therapy. Marine sponges have historically been a source of compounds with anticancer activity. In this study, we screened extracts from twenty marine sponges collected off the coast of Western Australia for cytotoxic activity against TNBC cells. One very active extract derived from the sponge Monanchora viridis was selected for bioactivity-guided fractionation. Through multiple steps of purification, we isolated a potent cytotoxic compound, which was identified as crambescidin 800 (C800). We found that C800 exhibited cytotoxic potency in a panel of breast cancer cells, of which TNBC and luminal cancer cell models were the most sensitive. In addition, C800 induced cell cycle arrest at the G2/M phase, resulting in a decline in the expression of cyclin D1, CDK4, and CDK6 in TNBC cells. This effect was associated with the inhibition of phosphorylation of Akt, NF-κB, and MAPK pathways, resulting in apoptosis in TNBC cells.

• Authors: Sumi Shrestha, Anabel Sorolla, Jane Fromont, Pilar Blancafort, Gavin R Flematti
• Journal: Marine drugs (Mar Drugs) Vol. 16 Issue 2 (Feb 08 2018) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 29419736 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Apoptosis Regulatory Proteins
• Spiro Compounds
• crambescidin 800
• Guanidine

Topics

• Animals
• Antineoplastic Agents (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (drug effects)
• Cell Cycle Checkpoints (drug effects)
• Cell Line, Tumor
• Down-Regulation (drug effects)
• Genes, cdc (drug effects)
• Guanidine (analogs & derivatives, isolation & purification, pharmacology)
• Humans
• Male
• Phosphorylation (drug effects)
• Porifera (chemistry)
• Signal Transduction (drug effects)
• Spiro Compounds (isolation & purification, pharmacology)
• Triple Negative Breast Neoplasms (drug therapy, pathology)