Abstract | BACKGROUND AND PURPOSE: EXPERIMENTAL APPROACH: We have combined electrophysiology with direct measurements of extracellular adenosine using microelectrode biosensors in rat hippocampal slices. KEY RESULTS: We found that ethanol has bidirectional actions on adenosine signalling: depressant concentrations of ethanol (50 mM) increased the basal extracellular concentration of adenosine under baseline conditions, leading to the inhibition of synaptic transmission, but it inhibited adenosine release during evoked seizure activity in brain slices. The reduction in activity-dependent adenosine release was in part produced by effects on NMDA receptors, although other mechanisms also appeared to be involved. Low concentrations of ethanol (10-15 mM) enhanced pathological network activity by selectively blocking activity-dependent adenosine release. CONCLUSIONS AND IMPLICATIONS:
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Authors | Victoria Hughes, Magnus J E Richardson, Mark J Wall |
Journal | British journal of pharmacology
(Br J Pharmacol)
Vol. 175
Issue 9
Pg. 1471-1485
(05 2018)
ISSN: 1476-5381 [Electronic] England |
PMID | 29361192
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2018 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society. |
Chemical References |
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Topics |
- Adenosine
(metabolism, physiology)
- Animals
- Biosensing Techniques
(methods)
- Dose-Response Relationship, Drug
- Ethanol
(pharmacology)
- Excitatory Postsynaptic Potentials
(physiology)
- Hippocampus
(drug effects, metabolism, physiology)
- Male
- Rats
- Seizures
(physiopathology)
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