Abstract |
As a unique member of the cadherin superfamily, T-cadherin (T-cad) has been demonstrated to be associated with gastric cancer (GC) prognosis. To elucidate the function of T-cad in GC in vitro, the present study firstly examined T-cad protein expression in normal and gastric cancer tissues and cell lines, and it was demonstrated to be significantly downregulated in gastric cancer samples compared with normal samples. Control and T-cad expression vectors were then transfected into the MGC8-03 and AGS GC cell lines. Utilizing MTT, clonogenic, flow cytometry, wound healing and Transwell invasion assays in addition to Western blotting, the present study demonstrated that the overexpression of T-cad suppressed GC cell growth and colony formation via cell cycle arrest at the G0/G1 phase via downregulating the expression of cyclin dependent kinase 4 and Cyclin D1. In addition, overexpression of T-cad significantly inhibited GC cell migration and invasion by increasing E-cadherin and decreasing Vimentin expression. These findings suggest T-cad may be important in GC cell proliferation and metastasis and serve as a promising target for the treatment of GC in the future.
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Authors | Jianqing Lin, Zhiyao Chen, Zhijun Huang, Feng Chen, Zeyi Ye, Shaoze Lin, Weidong Wang |
Journal | Experimental and therapeutic medicine
(Exp Ther Med)
Vol. 14
Issue 5
Pg. 4194-4200
(Nov 2017)
ISSN: 1792-0981 [Print] Greece |
PMID | 29104635
(Publication Type: Journal Article)
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